College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China.
College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Int J Mol Sci. 2017 Oct 1;18(10):2083. doi: 10.3390/ijms18102083.
Cadmium (Cd) and excess copper (Cu) are toxic to plants, causing a wide range of deleterious effects including the formation of reactive oxygen species. Metallothioneins (MTs) may protect plant cells from heavy metal toxicity by chelating heavy metals via cysteine thiol groups. They may also function as antioxidants. The study investigated the relationship of H₂O₂ production and expression in rice radicles and rice suspension cells under Cu or Cd stress. The results showed that H₂O₂ production in the rice radicles increased before Cu-induced expression, and after Cd-induced expression. Rice suspension cells of sense- and antisense- transgenic lines were obtained by an -mediated transformation. Overexpression of significantly decreased the death rate of rice cells, which was accompanied by blocked H₂O₂ accumulation in rice suspension cells subject to Cu and Cd stress. Our findings confirm that H₂O₂ is involved in the -mediated tolerance of Cu and Cd toxicity in rice.
镉 (Cd) 和过量的铜 (Cu) 对植物有毒，会导致一系列有害影响，包括活性氧的形成。金属硫蛋白 (MTs) 可以通过半胱氨酸硫醇基团螯合重金属来保护植物细胞免受重金属毒性的影响。它们也可能具有抗氧化剂的功能。本研究调查了在 Cu 或 Cd 胁迫下，水稻幼根和水稻悬浮细胞中 H₂O₂产生和表达的关系。结果表明，在 Cu 诱导表达之前，水稻幼根中 H₂O₂的产生增加，而在 Cd 诱导表达之后。通过介导的转化获得了 sense- 和 antisense- 转基因系的水稻悬浮细胞。 过表达 显著降低了水稻细胞的死亡率，伴随着在 Cu 和 Cd 胁迫下，水稻悬浮细胞中 H₂O₂积累的阻断。我们的研究结果证实 H₂O₂参与了水稻对 Cu 和 Cd 毒性的 介导的耐受性。
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