Silva Alex Fiori, Dos Santos Adriele Rodrigues, Coelho Trevisan Daliah Alves, Ribeiro Alessandra Braga, Zanetti Campanerut-Sá Paula Aline, Kukolj Caroline, de Souza Emanuel Maltempi, Cardoso Rosilene Fressatti, Estivalet Svidzinski Terezinha Inez, de Abreu Filho Benicio Alves, Junior Miguel Machinski, Graton Mikcha Jane Martha
Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Brasil, Avenida Colombo 5790, Maringá, Paraná, 87020-900, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Brasil, Avenida Cel. Francisco Santos, Curitiba, Paraná, 81531-980, Brazil.
Res Microbiol. 2018 Jan;169(1):33-43. doi: 10.1016/j.resmic.2017.09.007. Epub 2017 Sep 30.
The effect of cinnamaldehyde against biofilm cells of Salmonella Typhimurium ATCC 14028 was evaluated. We also assessed differential protein patterns that were expressed by biofilms compared with planktonic cells and protein expression by cinnamaldehyde-treated biofilms cells. This compound decreased biofilm biomass and metabolic activity of biofilms at both concentrations tested. Cinnamaldehyde treatment reduced the number of attached cells in polypropylene, reflected by colony count and scanning electron microscopy. The proteomic analysis of biofilms compared with planktonic cells indicated that several proteins were upregulated or downregulated, especially proteins that are involved in energy metabolism. Peroxiredoxin, ATP synthase alpha chain protein, conjugal transfer nickase/helicase TraI and elongation factor G were upregulated in untreated-biofilm cells, and their expression decreased as a function of cinnamaldehyde treatment. Cinnamaldehyde had antibiofilm activity, and several differentially expressed proteins identified provide potential and interesting targets to explore new control strategies for S. Typhimurium biofilms.
评估了肉桂醛对鼠伤寒沙门氏菌ATCC 14028生物膜细胞的作用。我们还评估了生物膜与浮游细胞相比所表达的差异蛋白质模式，以及肉桂醛处理的生物膜细胞的蛋白质表达。在两个测试浓度下，该化合物均降低了生物膜的生物量和生物膜的代谢活性。通过菌落计数和扫描电子显微镜反映，肉桂醛处理减少了聚丙烯中附着细胞的数量。与浮游细胞相比，生物膜的蛋白质组分析表明，几种蛋白质上调或下调，尤其是参与能量代谢的蛋白质。在未处理的生物膜细胞中，过氧化物还原酶、ATP合酶α链蛋白、接合转移切口酶/解旋酶TraI和延伸因子G上调，并且它们的表达随着肉桂醛处理而降低。肉桂醛具有抗生物膜活性，鉴定出的几种差异表达蛋白质为探索鼠伤寒沙门氏菌生物膜的新控制策略提供了潜在且有趣的靶点。
