promotes proliferation and inhibits apoptosis of cervical cancer cells via the Wnt signaling pathway.

Though the long noncoding RNA colon cancer associated transcript-1 () has been shown to be involved in tumors of other tissues, its involvement in cervical cancer is still unknown. Therefore, the aim of this study was to investigate the molecular mechanism of CCAT-1 in cervical cancer. We quantified the expression of long noncoding RNA in samples of cervical cancer tissue by real-time PCR. Effects of expression on the proliferation and apoptosis of HeLa and CaSki cells were assessed by cell-count, colony-formation, and flow cytometry assays. Binding of the c-Myc protein to the promoter was confirmed by chromatin immunoprecipitation. Finally, TOP-Flash and western blotting were used to examine the regulation of the Wnt/β-catenin pathway by . The results showed that compared with adjacent normal tissue, the expression of in cervical cancer tissue was significantly upregulated. expression was related to the stage and size of the tumor and recurrence prognosis. Then, we showed through functional assays that could promote proliferation and inhibit apoptosis of cervical cancer cells. Furthermore, chromatin immunoprecipitation showed that c-Myc protein could promote expression by binding to its promoter. Finally, fluorescent-reporter assays and western blotting showed that could activate the Wnt/β-catenin pathway. In conclusion, we showed that can be activated by the c-Myc protein and it can promote proliferation and inhibit apoptosis in cervical cancer cells by regulating the Wnt/β-catenin pathway. might serve as a good prognostic indicator and target for treatment of cervical cancer.