盐酸贝那普利通过降低血管生成素样蛋白4（ANGPTL-4）的表达来改善糖尿病肾病并减少蛋白尿。
Benazepril hydrochloride improves diabetic nephropathy and decreases proteinuria by decreasing ANGPTL-4 expression.
作者信息
Xue Lingyu, Feng Xiaoqing, Wang Chuanhai, Zhang Xuebin, Sun Wenqiang, Yu Kebo
机构信息
Department of Nephrology, the Affiliated Hospital of Taishan Medical University, No. 706 Taishan Street, Taian, Shandong Province, 271000, China.
出版信息
BMC Nephrol. 2017 Oct 4;18(1):307. doi: 10.1186/s12882-017-0724-1.
BACKGROUND
This study aimed to investigate the effects of benazepril hydrochloride (BH) on proteinuria and ANGPTL-4 expression in a diabetic nephropathy (DN) rat model.
METHODS
A total of 72 Wistar male rats were randomly divided into three groups: normal control (NC), DN group and BH treatment (BH) groups. The DN model was induced by streptozotocin (STZ). Weight, glucose, proteinuria, biochemical indicators and the kidney weight index were examined at 8, 12 and 16 weeks. In addition, ANGPTL-4 protein and mRNA expressions were assessed by immunohistochemistry and qRT-PCR, respectively. Relationships between ANGPTL-4 and biochemical indicators were investigated using Spearman analysis.
RESULTS
Weight was significantly lower but glucose levels were significantly higher in both the DN and BH groups than in the NC group (P < 0.05). Compared with the DN group, proteinuria, urea, creatinine, triglycerides and total cholesterol levels were decreased, whereas the albumin level was increased after BH treatment (all P < 0.05). Furthermore, BH diminished kidney volume and ameliorated the pathological changes associated with DN. ANGPTL-4 expression was significantly decreased after BH treatment, and ANGPTL-4 expression was highly correlated with biochemical indicators of DN (P < 0.05).
CONCLUSIONS
Benazepril hydrochloride improves DN and decreases proteinuria by decreasing ANGPTL-4 expression.
背景
本研究旨在探讨盐酸贝那普利（BH）对糖尿病肾病（DN）大鼠模型蛋白尿及血管生成素样蛋白4（ANGPTL-4）表达的影响。
方法
将72只雄性Wistar大鼠随机分为三组：正常对照组（NC）、DN组和BH治疗组。采用链脲佐菌素（STZ）诱导建立DN模型。在第8、12和16周时检测体重、血糖、蛋白尿、生化指标及肾脏重量指数。此外，分别采用免疫组织化学和qRT-PCR检测ANGPTL-4蛋白和mRNA表达。采用Spearman分析研究ANGPTL-4与生化指标之间的关系。
结果
DN组和BH组的体重均显著低于NC组，而血糖水平显著高于NC组（P<0.05）。与DN组相比，BH治疗后蛋白尿、尿素、肌酐、甘油三酯和总胆固醇水平降低，而白蛋白水平升高（均P<0.05）。此外，BH减小了肾脏体积，改善了与DN相关的病理变化。BH治疗后ANGPTL-4表达显著降低，且ANGPTL-4表达与DN的生化指标高度相关（P<0.05）。
结论
盐酸贝那普利通过降低ANGPTL-4表达改善DN并减少蛋白尿。
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