Wu Guanrong, Liu Baoyi, Wu Qiaowei, Tang Changting, Du Zijing, Fang Ying, Hu Yijun, Yu Honghua
School of Medicine, South China University of Technology, Guangzhou, China.
Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Front Med (Lausanne). 2021 Sep 28;8:727407. doi: 10.3389/fmed.2021.727407. eCollection 2021.
To investigate the expression of various angiogenesis and inflammation mediators in the vitreous fluid of eyes with proliferative diabetic retinopathy (PDR). A total of 38 eyes with PDR and 37 control eyes were included. Vitreous fluid was collected during vitrectomy. Vitreous levels of colony stimulating factor-1 receptor (CSF-1R), syndecan-1, placental growth factor (PIGF), and angiopoietin-like protein 4 (ANGPTL-4) were measured by multiplex immunoassay. Vitreous levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), interleukin-8 (IL-8), monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-α (TNF-), and intercellular adhesion molecule-1 (ICAM-1) were measured by cytometric beads array. Levels of these mediators were compared between the PDR and control eyes. Correlations between levels of different mediators and between these mediators and kidney function metrics in the PDR group were also analyzed. Vitreous levels of syndecan-1, PIGF, ANGPTL-4, VEGF, and IL-8 were significantly higher in the PDR group compared to the control group (all < 0.05). Levels of VEGF were significantly correlated with levels of syndecan-1, PIGF, and ANGPTL-4 ( = 0.370 to 0.497, all < 0.05). Significant positive correlations were detected between levels of any two of the following mediators including syndecan-1, PIGF, ANGPTL-4, and IL-8 ( = 0.370 to 0.906, all < 0.05). Apart from VEGF, levels of these mediators were positively correlated with serum creatinine and blood urea nitrogen ( = 0.328 to 0.638, all < 0.05), and negatively correlated with fasting blood glucose and estimated glomerular filtration rate ( = -0.325 to -0.603, all < 0.05). Correlations between different angiogenesis and inflammation mediators were observed in eyes with PDR, suggesting cross-talks of different angiogenesis and inflammation pathways in the pathogenesis of PDR. The levels of angiogenesis and inflammation in PDR are correlated with kidney damage, indicating possible common pathways in diabetic retinopathy and nephropathy.
研究增殖性糖尿病视网膜病变（PDR）患者眼玻璃体液中多种血管生成和炎症介质的表达。共纳入38例PDR患者的38只眼和37例对照眼。在玻璃体切割术中收集玻璃体液。采用多重免疫测定法检测集落刺激因子-1受体（CSF-1R）、多功能蛋白聚糖-1、胎盘生长因子（PIGF）和血管生成素样蛋白4（ANGPTL-4）的玻璃体水平。采用细胞计数珠阵列检测血管内皮生长因子（VEGF）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、单核细胞趋化蛋白-1（MCP-1）、肿瘤坏死因子-α（TNF-α）和细胞间黏附分子-1（ICAM-1）的玻璃体水平。比较PDR组和对照组这些介质的水平。还分析了PDR组中不同介质水平之间以及这些介质与肾功能指标之间的相关性。与对照组相比，PDR组的多功能蛋白聚糖-1、PIGF、ANGPTL-4、VEGF和IL-8的玻璃体水平显著更高（均P<0.05）。VEGF水平与多功能蛋白聚糖-1、PIGF和ANGPTL-4水平显著相关（r=0.370至0.497，均P<0.05）。在多功能蛋白聚糖-1、PIGF、ANGPTL-4和IL-8这些介质中的任意两种之间检测到显著的正相关（r=0.370至0.906，均P<0.05）。除VEGF外，这些介质的水平与血清肌酐和血尿素氮呈正相关（r=0.328至0.638，均P<0.05），与空腹血糖和估计肾小球滤过率呈负相关（r=-0.325至-0.603，均P<0.05）。在PDR患者眼中观察到不同血管生成和炎症介质之间的相关性，提示在PDR发病机制中不同血管生成和炎症途径之间存在相互作用。PDR中的血管生成和炎症水平与肾脏损伤相关，表明糖尿病视网膜病变和肾病可能存在共同途径。
