Ifeoluwa Okeleye Benjamin, Mkwetshana Noxolo Thabiso, Ndip Roland Ndip
Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, South Africa.
Phytomedicine and Phytopharmacology Research Group, Department of Plant Sciences, University of the Free State, Qwaqwa Campus, Phuthaditjhaba 9866, South Africa.
Iran J Pharm Res. 2017 Spring;16(2):714-724.
We evaluated the antiproliferative and apoptotic potential of the ethyl acetate extract (EAE) of , a member of the family Fabaceae (Sond) in order to validate its pharmacological use. Antiproliferation of human breast (MCF-7), colon (HT-29) and cervical (HeLa) cancer cell lines by EAE was investigated using the Cell Titer-Blue viability assay and the mechanism of action delineated using the Nucleic Acid and Protein Purification Nucleospin Tissue Kit, Scanning Electron Microscope (SEM), propidium iodide (PI) and acridine orange (AO) double-staining techniques. We observed a significant reduction in cell viability of the MCF-7 cells from 100% (untreated) to 54.33 ± 1.84% after 72 h of treatment with 5 µg/mL of EAE ( < 0.05). Internucleosomal DNA of MCF-7, HT-29 and HeLa cells was randomly fragmented into an uninterrupted spectrum of sizes, complemented by the intercalation of nucleic acid-specific fluorochromes by PI and AO spotting two phases of apoptosis; early (EA) and late (LA) apoptosis. Distinctive ultramorphological changes observed included; cell shrinkage, membrane blebbing, and typical cell induced death. The ethyl acetate extract of has the potential to induce apoptosis and is undergoing further studies as a likely template for new anticancer therapy.
我们评估了豆科（桑德）成员的乙酸乙酯提取物（EAE）的抗增殖和凋亡潜力，以验证其药理学用途。使用Cell Titer-Blue活力测定法研究了EAE对人乳腺癌（MCF-7）、结肠癌细胞（HT-29）和宫颈癌细胞（HeLa）的抗增殖作用，并使用核酸和蛋白质纯化Nucleospin组织试剂盒、扫描电子显微镜（SEM）、碘化丙啶（PI）和吖啶橙（AO）双重染色技术来描述其作用机制。我们观察到，在用5μg/mL的EAE处理72小时后，MCF-7细胞的活力从100%（未处理）显著降低至54.33±1.84%（P<0.05）。MCF-7、HT-29和HeLa细胞的核小体间DNA被随机片段化为连续的大小谱，PI和AO对核酸特异性荧光染料的嵌入补充了凋亡的两个阶段：早期（EA）和晚期（LA）凋亡。观察到的明显超微形态学变化包括：细胞收缩、膜泡化和典型的细胞诱导死亡。该植物的乙酸乙酯提取物具有诱导凋亡的潜力，作为一种可能的新型抗癌治疗模板正在进行进一步研究。
