Okeleye Benjamin I, Mkwetshana Noxolo T, Ndip Roland N
Microbial Pathogenicity and Molecular Epidemiology Research Group, Department of Biochemistry & Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa.
ScientificWorldJournal. 2013;2013:878735. doi: 10.1155/2013/878735. Epub 2013 Mar 11.
We assessed the in vitro antimicrobial activity of Peltophorum africanum by means of the agar well and macrodilution methods. The toxicity on a normal human liver cell (Chang liver cell) was determined using the CellTiter-Blue cell viability assay, and the compounds contained in the fractions were identified using GC-MS. Zone diameter of inhibition of the extract ranged from 12.5 ± 0.7 to 32 ± 2.8 mm for bacteria and from 7.5 ± 0.7 to 26.4 ± 3.4 mm for yeast. Marked activity of the extract was observed against Plesiomonas shigelloides ATCC 51903, with MIC and MLC values of 0.15625 and 0.3125 mg/mL, respectively. The extract was both bactericidal (MIC(index) ≤ 2) and bacteriostatic/fungistatic (MIC(index) > 2) in activity. Lethal dose at 50 (LD50) showed 82.64 ± 1.40 degree of toxicity at 24 hrs, and 95 percentile of cell death dose activity ranged from log 3.12 ± 0.01 to 4.59 ± 0.03. The activity of the eight fractions tested ranged from 1.0 ± 0.5 to 3.7 ± 1.6 mg/mL (IC50) and from 2.1 ± 0.8 to 6.25 ± 0 mg/mL (IC90). The extract was toxic to human Chang liver cell lines.
我们通过琼脂孔法和大稀释法评估了非洲盾柱木的体外抗菌活性。使用CellTiter-Blue细胞活力测定法测定了其对正常人肝细胞（张氏肝细胞）的毒性，并采用气相色谱-质谱联用仪（GC-MS）鉴定了各馏分中所含的化合物。提取物对细菌的抑菌圈直径范围为12.5±0.7至32±2.8毫米，对酵母的抑菌圈直径范围为7.5±0.7至26.4±3.4毫米。观察到提取物对类志贺邻单胞菌ATCC 51903具有显著活性，其最低抑菌浓度（MIC）和最低杀菌浓度（MLC）值分别为0.15625和0.3125毫克/毫升。提取物的活性兼具杀菌（MIC指数≤2）和抑菌/抑真菌（MIC指数>2）作用。半数致死剂量（LD50）显示，在24小时时毒性为82.64±1.40度，细胞死亡剂量活性的第95百分位数范围为log 3.12±0.01至4.59±0.03。所测试的八个馏分的活性范围为1.0±0.5至3.7±1.6毫克/毫升（IC50）和2.1±0.8至6·25±0毫克/毫升（IC90）。该提取物对人张氏肝癌细胞系有毒性。
