Zhao Yu, Zhou Donghui, Chen Jia, Sun Xiaolin
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Iran J Parasitol. 2017 Jul-Sep;12(3):332-338.
as a eukaryotic parasite of the phylum Apicomplexa, can infect almost all the warm-blooded animals and humans, causing toxoplasmosis. Rhoptry neck proteins (RONs) play a key role in the invasion process of and are potential vaccine candidate molecules against toxoplasmosis.
The present study examined sequence variation in the rhoptry neck protein 10 (TgRON10) gene among 10 isolates from different hosts and geographical locations from Lanzhou province during 2014, and compared with the corresponding sequences of strains ME49 and VEG obtained from the ToxoDB database, using polymerase chain reaction (PCR) amplification, sequence analysis, and phylogenetic reconstruction by Bayesian inference (BI) and maximum parsimony (MP).
Analysis of all the 12 TgRON10 genomic and cDNA sequences revealed 7 exons and 6 introns in the TgRON10 gDNA. The complete genomic sequence of the TgRON10 gene ranged from 4759 bp to 4763 bp, and sequence variation was 0-0.6% among the 12 isolates, indicating a low sequence variation in TgRON10 gene. Phylogenetic analysis of TgRON10 sequences showed that the cluster of the 12 isolates was not completely consistent with their respective genotypes.
TgRON10 gene is not a suitable genetic marker for the differentiation of isolates from different hosts and geographical locations, but may represent a potential vaccine candidate against toxoplasmosis, worth further studies.
作为顶复门的一种真核寄生虫，可感染几乎所有温血动物和人类，引发弓形虫病。棒状体颈部蛋白（RONs）在弓形虫的入侵过程中起关键作用，是抗弓形虫病潜在的疫苗候选分子。
本研究检测了2014年来自兰州不同宿主和地理位置的10株弓形虫分离株的棒状体颈部蛋白10（TgRON10）基因的序列变异，并与从ToxoDB数据库获得的ME49和VEG株的相应序列进行比较，采用聚合酶链反应（PCR）扩增、序列分析以及贝叶斯推断（BI）和最大简约法（MP）进行系统发育重建。
对所有12条TgRON10基因组和cDNA序列的分析显示，TgRON10基因DNA中有7个外显子和6个内含子。TgRON10基因的完整基因组序列长度在4759 bp至4763 bp之间，12株弓形虫分离株之间的序列变异为0 - 0.6%，表明TgRON10基因的序列变异较低。TgRON10序列的系统发育分析表明，12株弓形虫分离株的聚类与其各自的基因型并不完全一致。
TgRON10基因不是区分不同宿主和地理位置的弓形虫分离株的合适遗传标记，但可能是一种抗弓形虫病的潜在疫苗候选物，值得进一步研究。
