Czech Academy of Sciences, Institute of Analytical Chemistry, Brno, Czech Republic.
Masaryk University, Faculty of Science, Brno, Czech Republic.
J Sep Sci. 2018 Jan;41(1):236-247. doi: 10.1002/jssc.201700878. Epub 2017 Oct 30.
Progress achieved between 2014-2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.
本文综述了 2014-2017 年等电聚焦在核酸提取和样品制备方面的进展。由于血液和其他体液、犯罪现场样本、化石样本等复杂基质的存在，核酸的分离和纯化常常受到影响。虽然大多数常见的核酸分离技术都是基于具有定量结果固有局限性的提取，但等电聚焦聚焦是一种定量过程，具有理论上无限的浓缩因子。由于等电聚焦属于核酸纯化的较传统方法，因此我们不仅介绍了等电聚焦在核酸浓缩方面的最新应用进展，还简要描述了该方法的原理。
