Li Zhao, Zhao Jiang-Lin, Wu Yu-Tian, Mu Lan, Zeng Xi, Jin Zongwen, Wei Gang, Xie Ning, Redshaw Carl
Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province; School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.
Org Biomol Chem. 2017 Oct 18;15(40):8627-8633. doi: 10.1039/c7ob02301d.
A tripodal fluorescent probe L1 armed with Rhodamine B and 1-naphthaleneisothiocyanates was prepared in high yield. A study of the recognition properties revealed that probe L1 exhibited high sensitivity and selectivity towards Al through a "FRET" fluorescence response and colorimetric response with low detection limits of the order of 10 M. Meanwhile, probe L1 also possessed high recognition ability for I through fluorescence decay, which given there are comparatively few selective fluorescent probes for I, is significant. Furthermore, the complexation mechanisms were fully investigated by spectral titrations, H NMR spectroscopic titrations and mass spectrometry. The utility of probe L1 as a biosensor in living cells (PC3 cells) towards Al ions has also been demonstrated.
制备了一种带有罗丹明B和1-萘异硫氰酸酯的三脚架荧光探针L1，产率很高。对其识别性能的研究表明，探针L1通过“荧光共振能量转移（FRET）”荧光响应和比色响应，对铝表现出高灵敏度和选择性，检测限低至10⁻⁹ M量级。同时，探针L1对碘离子也具有通过荧光衰减的高识别能力，鉴于针对碘离子的选择性荧光探针相对较少，这一点意义重大。此外，通过光谱滴定、¹H NMR光谱滴定和质谱对络合机制进行了全面研究。还证明了探针L1作为生物传感器在活细胞（PC3细胞）中对铝离子的实用性。
