三氧化二砷通过上调乳腺癌细胞中的 let-7a 抑制细胞生长和迁移。
Arsenic trioxide inhibits cell growth and motility via up-regulation of let-7a in breast cancer cells.
机构信息
a Department of Biochemistry and Molecular Biology , Bengbu Medical College, Bengbu , Anhui , China.
b Research Center of Clinical Laboratory Science , Bengbu Medical College , Bengbu , Anhui , China.
出版信息
Cell Cycle. 2017;16(24):2396-2403. doi: 10.1080/15384101.2017.1387699. Epub 2017 Nov 20.
Abstract
Arsenic trioxide (ATO) has been reported to exert its anti-cancer activities in human cancers. However, the molecular mechanism of ATO-triggered anti-tumor activity has not been fully elucidated. Recently, multiple studies demonstrated that ATO could regulate miRNAs in human cancers. Therefore, in this study, we investigated whether ATO regulated let-7a in breast cancer cells. We found that ATO upregulated let-7a level in breast cancer cells. We also found that up-regulation of let-7a inhibited cell growth and induced apoptosis and retarded cell migration and invasion. We also observed that up-regulation of let-7a enhanced cell growth inhibition and invasion suppression induced by ATO treatment. Our findings suggest that ATO suppressed cell growth, stimulated apoptosis, and retarded cell invasion partly via upregulation of let-7a in breast cancer cells. Our study provides a new anti-tumor mechanism of ATO treatment in breast cancer.
摘要
三氧化二砷（ATO）已被报道在人类癌症中发挥抗癌作用。然而，ATO 触发抗肿瘤活性的分子机制尚未完全阐明。最近，多项研究表明 ATO 可以调节人类癌症中的 miRNAs。因此，在本研究中，我们研究了 ATO 是否调节乳腺癌细胞中的 let-7a。我们发现 ATO 上调了乳腺癌细胞中的 let-7a 水平。我们还发现，let-7a 的上调抑制了细胞生长并诱导了细胞凋亡，减缓了细胞迁移和侵袭。我们还观察到，let-7a 的上调增强了 ATO 处理诱导的细胞生长抑制和侵袭抑制。我们的研究结果表明，ATO 通过上调乳腺癌细胞中的 let-7a 抑制细胞生长、刺激细胞凋亡和减缓细胞侵袭。我们的研究为 ATO 治疗乳腺癌提供了一种新的抗肿瘤机制。
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本文引用的文献
1
Extracellular signal-regulated kinase 8-mediated NF-κB activation increases sensitivity of human lung cancer cells to arsenic trioxide.
Oncotarget. 2017 Jul 25;8(30):49144-49155. doi: 10.18632/oncotarget.17100.
2
High-dose ascorbate and arsenic trioxide selectively kill acute myeloid leukemia and acute promyelocytic leukemia blasts in vitro.
Oncotarget. 2017 May 16;8(20):32550-32565. doi: 10.18632/oncotarget.15925.
3
Retinoic acid and arsenic trioxide in the treatment of acute promyelocytic leukemia: current perspectives.
Onco Targets Ther. 2017 Mar 14;10:1585-1601. doi: 10.2147/OTT.S100513. eCollection 2017.
4
Demethylation and alterations in the expression level of the cell cycle-related genes as possible mechanisms in arsenic trioxide-induced cell cycle arrest in human breast cancer cells.
Tumour Biol. 2017 Feb;39(2):1010428317692255. doi: 10.1177/1010428317692255.
5
Combination of Arsenic trioxide and Everolimus (Rad001) synergistically induces both autophagy and apoptosis in prostate cancer cells.
Oncotarget. 2017 Feb 14;8(7):11206-11218. doi: 10.18632/oncotarget.14493.
6
Cancer Statistics, 2017.
CA Cancer J Clin. 2017 Jan;67(1):7-30. doi: 10.3322/caac.21387. Epub 2017 Jan 5.
7
Let-7 Status Is Crucial for PARP1 Expression in HER2-Overexpressing Breast Tumors.
Mol Cancer Res. 2017 Mar;15(3):340-347. doi: 10.1158/1541-7786.MCR-16-0287-T. Epub 2016 Dec 28.
8
Micro RNA 34a and Let-7a Expression in Human Breast Cancers is Associated with Apoptotic Expression Genes.
Asian Pac J Cancer Prev. 2016;17(4):1887-90. doi: 10.7314/apjcp.2016.17.4.1887.
9
MicroRNA-206, let-7a and microRNA-21 pathways involved in the anti-angiogenesis effects of the interval exercise training and hormone therapy in breast cancer.
Life Sci. 2016 Apr 15;151:30-40. doi: 10.1016/j.lfs.2016.02.090. Epub 2016 Feb 26.
10
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Biomed Pharmacother. 2016 Mar;78:301-307. doi: 10.1016/j.biopha.2016.01.028. Epub 2016 Feb 4.
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