Rose M E, Joysey H S, Hesketh P, Grencis R K, Wakelin D
Houghton Laboratory, Institute for Animal Health, Huntingdon, United Kingdom.
Infect Immun. 1988 Jul;56(7):1760-5. doi: 10.1128/iai.56.7.1760-1765.1988.
Immunity to infection with Eimeria vermiformis was transferred in NIH mice by both the nylon wool-adherent (B-cell-enriched) and nonadherent (T-cell-enriched) fractions of lymphocytes (spleen and mesenteric lymph node) taken from infected donors. Transfer was more variable with the adherent fraction, and when contaminating T cells were removed by treatment with anti-Thy1 monoclonal antibody (MAb) and complement, this fraction lost all protective activity. The protective effect of T-cell-enriched populations of mesenteric lymphocytes was abrogated by treatment with anti-L3T4 MAb and complement in vitro before transfer or by opsonization with this MAb in vitro before intravenous inoculation into recipients. Similar treatments of cells with anti-Lyt2 MAb did not have this effect, confirming that Thy1+ L3T4+ cells mediate the adoptive transfer of immunity to E. vermiformis. Thy1+ L3T4+ cells were also shown to limit the replication of E. vermiformis in primary infections: mice depleted of this subset (by thymectomy followed by intravenous injection of anti-L3T4 MAb) passed greater numbers of oocysts over a longer period of time than did mice similarly depleted of Lyt2+ cells.
将感染了蠕虫艾美耳球虫的供体小鼠脾脏和肠系膜淋巴结淋巴细胞的尼龙毛黏附（富含B细胞）和非黏附（富含T细胞）部分，转移至NIH小鼠体内后，这些小鼠获得了对蠕虫艾美耳球虫感染的免疫力。黏附部分的转移效果更具变异性，当用抗Thy1单克隆抗体（MAb）和补体处理以去除污染的T细胞时，该部分失去了所有保护活性。在转移前体外先用抗L3T4 MAb和补体处理肠系膜淋巴细胞的富含T细胞群体，或在静脉接种受体前体外先用该MAb调理这些细胞，均可消除其保护作用。用抗Lyt2 MAb对细胞进行类似处理则没有这种效果，这证实了Thy1⁺L3T4⁺细胞介导了对蠕虫艾美耳球虫免疫力的过继转移。Thy1⁺L3T4⁺细胞也被证明在原发性感染中可限制蠕虫艾美耳球虫的复制：与同样去除Lyt2⁺细胞的小鼠相比，去除该亚群（通过胸腺切除术后静脉注射抗L3T4 MAb）的小鼠在更长时间内排出了更多数量的卵囊。
