Rose M E, Wakelin D, Joysey H S, Hesketh P
Houghton Poultry Research Station, Huntingdon, Cambridgeshire, UK.
Parasite Immunol. 1988 Jan;10(1):59-69. doi: 10.1111/j.1365-3024.1988.tb00203.x.
The development of a reliable model for the adoptive transfer of immunity to coccidiosis (infection with Eimeria vermiformis in NIH mice) is described. More than 10(8) of a mixture of spleen and mesenteric lymph node (MLN) cells, given either intravenously or intraperitoneally, were required to transfer a significant degree of protection. Dividing cells, present in the donors at 10 or 14 days after priming, but not at 5 or 19 days, were shown to be the effectors. When examined separately, MLN cells were found to be superior to spleen cells, and the injection of as few as 5 x 10(7) was capable of substantially reducing the oocyst output from a challenge inoculum. The recipients of cells from primed mice had earlier, and sometimes higher, titres of specific antibodies in the serum but, overall, there was no correlation between these titres and protection. Further characterization of the cells responsible for adoptively transferring immunity to this infection should now be possible.
本文描述了一种用于将抗球虫病免疫力（NIH小鼠感染蠕形艾美耳球虫）进行过继转移的可靠模型的建立。通过静脉或腹腔注射超过10⁸个脾细胞和肠系膜淋巴结（MLN）细胞的混合物，才能转移显著程度的保护作用。在致敏后10天或14天供体中存在的分裂细胞，但在5天或19天时不存在，被证明是效应细胞。单独检查时，发现MLN细胞优于脾细胞，注射低至5×10⁷个细胞就能显著减少攻击接种物的卵囊产量。来自致敏小鼠的细胞受体血清中特异性抗体的滴度更早出现，有时更高，但总体而言，这些滴度与保护作用之间没有相关性。现在应该能够进一步鉴定负责过继转移这种感染免疫力的细胞。
