Lee Yunho, Jeon Che Ok
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4767-4772. doi: 10.1099/ijsem.0.002377. Epub 2017 Oct 6.
A Gram-stain-positive, facultatively aerobic and endospore-forming bacterium, designated strain Pch-40, was isolated from a freshwater green alga, Paulinella chromatophora. Cells were motile rods with a monotrichous polar flagellum showing catalase- and oxidase-positive reactions. Strain Pch-40 grew at 20-50 °C (optimum, 37-40 °C), at pH 5.0-11.0 (optimum, pH 7.0) and in the presence of 0-4.0 % (w/v) NaCl (optimum, 0 %). Menaquinone-7 was detected as the sole isoprenoid quinone. The genomic DNA G+C content of strain Pch-40 was 55.6 mol%. The major cellular fatty acids of strain Pch-40 were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Pch-40 clearly belonged to the genus Cohnella of the family Paenibacillaceae. Strain Pch-40 was most closely related to Cohnella rhizosphaerae CSE-5610 with a 96.1 % 16S rRNA gene sequence similarity. The phenotypic and chemotaxonomic features and the phylogenetic inference clearly suggested that strain Pch-40 represents a novel species of the genus Cohnella, for which the name Cohnellaalgarum sp. nov. is proposed. The type strain is strain Pch-40 (=KACC 19279=JCM 32033).
从淡水绿藻——嗜盐色球藻（Paulinella chromatophora）中分离出一株革兰氏阳性、兼性需氧且形成芽孢的细菌，命名为菌株Pch-40。细胞为具单生极鞭毛的能动杆菌，过氧化氢酶和氧化酶反应呈阳性。菌株Pch-40在20-50 °C（最适温度为37-40 °C）、pH 5.0-11.0（最适pH为7.0）以及0-4.0 %（w/v）NaCl（最适浓度为0 %）的条件下生长。检测到甲基萘醌-7为唯一的类异戊二烯醌。菌株Pch-40的基因组DNA G+C含量为55.6 mol%。菌株Pch-40的主要细胞脂肪酸为C16 : 0、异-C16 : 0、anteiso-C15 : 0和anteiso-C17 : 0。主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。基于16S rRNA基因序列的系统发育分析表明，菌株Pch-40明显属于芽孢杆菌科（Paenibacillaceae）的考氏菌属（Cohnella）。菌株Pch-40与根际考氏菌（Cohnella rhizosphaerae）CSE-5610关系最为密切，16S rRNA基因序列相似性为96.1 %。表型、化学分类特征以及系统发育推断明确表明，菌株Pch-40代表考氏菌属的一个新物种，为此提出新名称考氏藻菌（Cohnellaalgarum sp. nov.）。模式菌株为菌株Pch-40（=KACC 19279=JCM 32033）。
