Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):83-87. doi: 10.1099/ijs.0.050849-0. Epub 2013 Sep 10.
A bacterial strain designated RLAHU4B(T) was isolated from root nodules of Lupinus albus in León (Spain). The 16S rRNA gene sequence of this strain showed similarities lower than 97 % with respect to species of the genus Cohnella. The strain was a Gram-variable, sporulating rod, motile by means of peritrichous flagella, and facultatively anaerobic. It was positive for oxidase, catalase and β-galactosidase production but negative for urease, amylase and gelatinase. Strain RLAHU4B(T) grew in the presence of 5 % NaCl. MK-7 was the predominant menaquinone and meso-diaminopimelic acid was present in the peptidoglycan. anteiso-C15 : 0, iso-C16 : 0, iso-C15 : 0 and C16 : 0 were the major fatty acids. Major polar lipids of strain RLAHU4B(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, two unknown aminophospholipids and one unknown lipid. The DNA G+C content was 57.8 mol%. Strain RLAHU4B(T) presented phenotypic differences from all recognized species of the genus Cohnella. The phylogenetic, chemotaxonomic and phenotypic data indicated that strain RLAHU4B(T) belongs to a novel species of the genus Cohnella, for which the name Cohnella lupini sp. nov. is proposed, with strain RLAHU4B(T) ( = LMG 27416(T) = CECT 8236(T)) as the type strain.
一株名为 RLAHU4B(T) 的细菌菌株从西班牙莱昂的白 Lupinus albus 根瘤中分离得到。该菌株的 16S rRNA 基因序列与 Cohnella 属的种的相似性低于 97%。该菌株为革兰氏可变、产芽孢的杆状菌，通过周生鞭毛运动，兼性厌氧。它氧化酶、过氧化氢酶和β-半乳糖苷酶产生产阳性，但脲酶、淀粉酶和明胶酶产生产阴性。菌株 RLAHU4B(T) 在 5%NaCl 存在的情况下生长。MK-7 是主要的menaquinone，肽聚糖中存在 meso-diaminopimelic 酸。anteiso-C15:0、iso-C16:0、iso-C15:0 和 C16:0 是主要的脂肪酸。菌株 RLAHU4B(T) 的主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未知磷脂、两种未知氨基磷脂和一种未知脂。DNA G+C 含量为 57.8 mol%。菌株 RLAHU4B(T) 在表型上与 Cohnella 属的所有公认种都有区别。系统发育、化学分类和表型数据表明，菌株 RLAHU4B(T) 属于 Cohnella 属的一个新种，建议命名为 Cohnella lupini sp. nov.，其模式株为 RLAHU4B(T) ( = LMG 27416(T) = CECT 8236(T))。
