Wang Chunling, Mao Lutian, Bao Gegen, Zhu Honghui
College of Life Science, Huizhou University, Huizhou 516007, China.
Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Microorganisms. 2023 Nov 8;11(11):2726. doi: 10.3390/microorganisms11112726.
Two strains, designated NL03-T5 and NL03-T5-1, were isolated from a soil sample collected from the Nanling National Forests, Guangdong Province, PR China. The two strains were Gram-stain-positive, aerobic, rod-shaped and had lophotrichous flagellation. Strain NL03-T5 could secrete extracellular mucus whereas NL03-T5-1 could not. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains belong to the genus , were most closely related to LMG 27416 (95.9% and 96.1% similarities), and both showed 94.0% similarity with NRRL B-59459, respectively. The two strains showed 99.8% 16S rRNA gene sequence similarity between them. The draft genome size of strain NL03-T5 was 7.44 Mbp with a DNA G+C content of 49.2 mol%. The average nucleotide identities (ANI) and the digital DNA-DNA hybridization (dDDH) values between NL03-T5 and NL03-T5-1 were 99.98% and 100%, indicating the two strains were of the same species. Additionally, the ANI and dDDH values between NL03-T5 and LMG 27416 were 76.1% and 20.4%, respectively. The major cellular fatty acids of strain NL03-T5 included anteiso-C and iso-C. The major polar lipids and predominant respiratory quinone were diphosphatidylglycerol (DPG) and menaquinone-7 (MK-7). Based on phylogenetic analysis, phenotypic and chemotaxonomic characterization, genomic DNA G+C content, and ANI and dDDH values, strains NL03-T5 and NL03-T5-1 represent novel species in the genus , for which the name is proposed. The type strain is NL03-T5 (=GDMCC 1.2294 = JCM 34999). Furthermore, comparative genomics revealed that the genus had an open pan-genome. The pan-genome of 29 strains contained 41,356 gene families, and the number of strain-specific genes ranged from 6 to 1649. The results may explain the good adaptability of the strains to different habitats at the genetic level.
从中国广东省南岭国家森林公园采集的土壤样本中分离出两株菌株，分别命名为NL03-T5和NL03-T5-1。这两株菌株革兰氏染色阳性，好氧，杆状，具束状鞭毛。菌株NL03-T5能分泌细胞外黏液，而NL03-T5-1不能。基于16S rRNA基因序列的系统发育分析表明，这两株菌株属于 属，与LMG 27416关系最为密切（相似性分别为95.9%和96.1%），且与NRRL B-59459的相似性均为94.0%。这两株菌株之间的16S rRNA基因序列相似性为99.8%。菌株NL03-T5的基因组草图大小为7.44 Mbp，DNA G+C含量为49.2 mol%。NL03-T5与NL03-T5-1之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为99.98%和100%，表明这两株菌株属于同一物种。此外，NL03-T5与LMG 27416之间的ANI和dDDH值分别为76.1%和20.4%。菌株NL03-T5的主要细胞脂肪酸包括anteiso-C和iso-C。主要极性脂类和主要呼吸醌分别为双磷脂酰甘油（DPG）和甲基萘醌-7（MK-7）。基于系统发育分析、表型和化学分类特征、基因组DNA G+C含量以及ANI和dDDH值，菌株NL03-T5和NL03-T5-1代表了 属中的新物种，为此提出名称 。模式菌株为NL03-T5（=GDMCC 1.2294 = JCM 34999）。此外，比较基因组学研究表明 属具有开放的泛基因组。29株 菌株的泛基因组包含41,356个基因家族，菌株特异性基因数量从6到1649不等。这些结果可能在遗传水平上解释了 菌株对不同生境的良好适应性。
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