Shi Huai, Rao Manik Prabhu Narsing, Chen Qian-Qian, Quadri Syed Raziuddin, Li Wen-Jun, Liu Guo-Hong
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou City, 350003, Fujian Province, PR China.
Facultad de Ingeniería, Instituto de Ciencias Aplicadas, Universidad Autónoma de Chile, 3460000, Sede Talca, Talca, Chile.
Curr Microbiol. 2025 Mar 12;82(5):185. doi: 10.1007/s00284-025-04161-w.
Two Gram-stain-positive, aerobic, rod-shaped, and motile strains FJAT-53684 and FJAT-54145 were isolated from the mangrove sediment. They optimally grew at pH 8.0 and could tolerate NaCl up to 5% (w/v). The optimum temperature for growth of strains FJAT-53684 and FJAT-54145 were 35 and 40 ºC, respectively. The 16S rRNA gene sequence similarity between FJAT-53684 and FJAT-54145 was 97.1%. Both strains showed the highest 16S rRNA gene sequence similarity to the Cytobacillus species. Menaquinone-7 was the only respiratory quinone present in both strains. The major fatty acids (≥ 10%) in strain FJAT-53684 were iso-C, anteiso-C, iso-C, and iso-C, while strain FJAT-54145 consist of iso-C, iso-C, iso-C, iso-C, and C ω7c alcohol. The polar lipids present in both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified lipid, unidentified phospholipid, and phosphatidylinositol. Their average nucleotide identity and digital DNA-DNA hybridization values were below the threshold values (95% and 70%, respectively) for species delineation. Based on the above results, strains FJAT-53684 and FJAT-54145 represent two novel species of the genus Cytobacillus, for which the names Cytobacillus mangrovibacter sp. nov., and Cytobacillus spartinae sp. nov., are proposed. The type strains are FJAT-53684 (= JCM 35618 = GDMCC 1.3071), and FJAT-54145 (= JCM 35621 = GDMCC 1.3079).
从红树林沉积物中分离出两株革兰氏阳性、需氧、杆状且具运动性的菌株FJAT - 53684和FJAT - 54145。它们在pH 8.0时生长最佳，且能耐受高达5%（w/v）的NaCl。菌株FJAT - 53684和FJAT - 54145生长的最适温度分别为35和40℃。FJAT - 53684和FJAT - 54145之间的16S rRNA基因序列相似性为97.1%。两株菌与芽孢杆菌属物种的16S rRNA基因序列相似性最高。甲基萘醌 - 7是两株菌中唯一存在的呼吸醌。菌株FJAT - 53684中的主要脂肪酸（≥10%）为异-C、anteiso-C、异-C和异-C，而菌株FJAT - 54145由异-C、异-C、异-C、异-C和C ω7c醇组成。两株菌中存在的极性脂质为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、未鉴定脂质、未鉴定磷脂和磷脂酰肌醇。它们的平均核苷酸一致性和数字DNA - DNA杂交值低于物种划分的阈值（分别为95%和70%）。基于上述结果，菌株FJAT - 53684和FJAT - 54145代表芽孢杆菌属的两个新物种，分别命名为红树林芽孢杆菌（Cytobacillus mangrovibacter sp. nov.）和 Spartinae芽孢杆菌（Cytobacillus spartinae sp. nov.）。模式菌株分别为FJAT - 53684（=JCM 35618 = GDMCC 1.3071）和FJAT - 54145（=JCM 35621 = GDMCC 1.3079）。
