Williams Michelle, Martin Jennifer, Galettis Peter
University of Newcastle, Faculty of Health and Medicine, University Drive, Callaghan, New South Wales, Australia.
J Anal Toxicol. 2017 Oct 1;41(8):659-669. doi: 10.1093/jat/bkx055.
Workplace drug testing in Australia is usually adherent to one of two standards, AS/NZS 4308:2008 for urine or AS 4760:2006 for oral fluid. These standards prescribe the drugs tested, devices used and testing methodology followed by the testing agency. However, they are not comprehensive and for many years workers have been able to consume novel psychoactive substances to avoid detection and without consequences. Here, we present a validated method for the detection of 32 Synthetic Stimulant and Hallucogenic drugs, commonly sold as bath salts, in oral fluid. These drugs are cathinone, ephedrone, methylone, flephedrone, MDA, PMA, methedrone, TMA, MDMA, butylone, mephedrone, MDEA, MEC, pentedrone, MBDB, MTA, Alpha-PVP, MPBP, 2C-B, MDPV, DOB, 2C-T-2, TFMPP, DOET, 2C-T-7, naphyrone, MDAI, FMA, DMA, 25C-NBOMe, 25B-NBOMe and 25T4-NBOMe. Sample preparation was undertaken using a simple protein precipitation in acetonitrile. Chromatographic separation was achieved in 7.5 min on a Kinetex F5 column (50 mm × 3 mm × 2.6 μm) using 0.1% formic acid in water and acetonitrile as the mobile phases. The method was validated with limit of detection (1 ng/mL), limit of quantitation (2.5 ng/mL), selectivity, linearity (2.5-500 ng/mL), accuracy (85.3-108.4% of the target concentration) and precision (1.9-14%). This method was applied to 12 samples previously submitted for routine testing and two were found to contain 2-CB and DOB (5 and 4 ng/mL) and, MPBP and TFMPP (both at 4 ng/mL). This method provides for the rapid detection of a large number of compounds in oral fluid which is readily applicable to routine testing laboratories.
澳大利亚的职场药物检测通常遵循两个标准之一，尿液检测遵循AS/NZS 4308:2008，口腔液检测遵循AS 4760:2006。这些标准规定了检测的药物、使用的设备以及检测机构遵循的检测方法。然而，它们并不全面，多年来，工人们一直能够服用新型精神活性物质来避免被检测到且无需承担后果。在此，我们提出了一种经过验证的方法，用于检测口腔液中32种通常作为浴盐出售的合成兴奋剂和致幻药物。这些药物包括卡西酮、麻黄酮、甲酮、氟麻黄酮、MDA、PMA、去甲伪麻黄碱、TMA、MDMA、丁酮、甲氧麻黄酮、MDEA、MEC、戊酮、MBDB、MTA、α-PVP、MPBP、2C-B、MDPV、DOB、2C-T-2、TFMPP、DOET、2C-T-7、萘黄酮、MDAI、FMA、DMA、25C-NBOMe、25B-NBOMe和25T4-NBOMe。样品制备采用在乙腈中进行简单的蛋白质沉淀。使用Kinetex F5柱（50 mm×3 mm×2.6μm），以0.1%甲酸水溶液和乙腈作为流动相，在7.5分钟内实现色谱分离。该方法通过检测限（1 ng/mL）、定量限（2.5 ng/mL）、选择性、线性（2.5 - 500 ng/mL）、准确度（目标浓度的85.3 - 108.4%）和精密度（1.9 - 14%）进行了验证。该方法应用于之前提交进行常规检测的12个样品，发现其中两个含有2-CB和DOB（分别为5和4 ng/mL）以及MPBP和TFMPP（均为4 ng/mL）。该方法可快速检测口腔液中的大量化合物，易于应用于常规检测实验室。
