Zhao M M, Gong D Q, Gao T, Zhang L, Li J L, Lv P A, Yu L L, Gao F, Zhou G H
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
J Anim Physiol Anim Nutr (Berl). 2018 Apr;102(2):e758-e767. doi: 10.1111/jpn.12831. Epub 2017 Oct 7.
The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the growth performance, energy reserves and mRNA expression levels of gluconeogenesis and glycogenesis enzymes in liver of late-term embryos and neonatal broilers were investigated. After candling on 16 day of incubation, a total of 960 eggs were randomly assigned to three treatments: (i) non-injected control, (ii) saline group injected with 0.6 ml of 0.75% physiological saline and (iii) Creatine pyruvate group injected with 0.6 ml of physiological saline containing 12 mg CrPyr/egg. After hatching, 120 male chicks with average body weight (BW) were randomly allocated into each treatment group for a 7-day feeding trial. The results showed that broilers subjected to CrPyr treatment had higher BW than those of the control and saline groups on 1, 3 and 7 day post-hatch, as well as the yolk sac weight on 19 day of incubation (19 E), the day of hatch and 3 day post-hatch (p < .05). Compared with the control and saline groups, IOF of CrPyr increased the plasma creatine concentration on the day of hatch, and the plasma pyruvate concentration on the day of hatch and 3 day post-hatch (p < .05). Moreover, IOF of CrPyr increased the liver pyruvate and glucose concentrations on 19 E and the day of hatch, and the liver glycogen concentration during the experiment (p < .05). Broilers in the CrPyr group showed increased mRNA expression levels of pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen synthase 2 (GYS2) on 19 E and the day of hatch (p < .05). These results indicated that IOF of CrPyr increased energy reserves in liver of embryos and neonatal broilers possibly through upregulating the mRNA expression levels of PC, PEPCK and GYS2, which could benefit the increase of BW in broilers on 7 day post-hatch.
研究了对晚期胚胎和新生肉鸡肝脏中丙酮酸肌酸（CrPyr）进行胚内注射（IOF）对其生长性能、能量储备以及糖异生和糖原生成酶mRNA表达水平的影响。在孵化第16天进行照蛋后，将总共960枚鸡蛋随机分为三组处理：（i）未注射对照组，（ii）注射0.6毫升0.75%生理盐水的生理盐水组，以及（iii）注射0.6毫升含12毫克CrPyr/枚鸡蛋的生理盐水的丙酮酸肌酸组。孵化后，将120只平均体重（BW）的雄性雏鸡随机分配到每个处理组进行为期7天的饲养试验。结果表明，接受CrPyr处理的肉鸡在孵化后第1、3和7天的体重高于对照组和生理盐水组，在孵化第19天（19 E）、出雏当天和出雏后第3天的卵黄囊重量也更高（p < 0.05）。与对照组和生理盐水组相比，CrPyr的IOF增加了出雏当天的血浆肌酸浓度，以及出雏当天和出雏后第3天的血浆丙酮酸浓度（p < 0.05）。此外，CrPyr的IOF增加了19 E和出雏当天肝脏中的丙酮酸和葡萄糖浓度，以及实验期间肝脏中的糖原浓度（p < 0.05）。CrPyr组的肉鸡在19 E和出雏当天丙酮酸羧化酶（PC）、磷酸烯醇式丙酮酸羧激酶（PEPCK）和糖原合酶2（GYS2）的mRNA表达水平升高（p < 0.05）。这些结果表明，CrPyr的IOF可能通过上调PC、PEPCK和GYS2的mRNA表达水平来增加胚胎和新生肉鸡肝脏中的能量储备，这可能有利于提高孵化后第7天肉鸡的体重。
