College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control , Nanjing Agricultural University , Nanjing 210095 , P. R. China.
College of Animal Science and Technology , Yangzhou University , Yangzhou 225009 , P. R. China.
J Agric Food Chem. 2018 Jul 25;66(29):7684-7691. doi: 10.1021/acs.jafc.8b02557. Epub 2018 Jul 12.
This study aims to investigate in ovo feeding (IOF) of creatine pyruvate (CrPyr) on glucose metabolism, hormone concentration, and the 5'-AMP-activated protein kinase (AMPK) pathway in breast muscle of embryos and neonatal broilers. The three treatments were noninjected control, 0.75% NaCl treatment, and 12 mg CrPyr/egg treatment. The solution was injected on the 17.5 day of incubation. At hatch, 120 male broilers from each treatment were chosen for a 7 day feeding trial. Compared with other treatments, CrPyr treated broilers enhanced insulin and thyroxine levels in plasma, adenosine triphosphate (ATP) concentration, hexokinase and pyruvate kinase activities, glucose transporter protein mRNA expressions, as well as protein abundances of phosphor-liver kinase B1 and phosphor-AMPK in breast muscle at hatch. In conclusion, IOF of CrPyr improved the energy status, increased the gene expression of glucose transporter proteins, and facilitated glycolysis in breast muscle, which may be associated with the activated AMPK pathway.
本研究旨在探讨胚胎期肌内注射肌酸丙酮酸（CrPyr）对葡萄糖代谢、激素浓度和 5'-AMP 激活蛋白激酶（AMPK）途径的影响。三个处理组分别为非注射对照组、0.75%NaCl 处理组和 12mg CrPyr/卵处理组。该溶液于孵化第 17.5 天注射。孵化时，每个处理组选择 120 只雄性肉鸡进行为期 7 天的饲养试验。与其他处理组相比，CrPyr 处理的肉鸡提高了血浆中的胰岛素和甲状腺素水平、三磷酸腺苷（ATP）浓度、己糖激酶和丙酮酸激酶活性、葡萄糖转运蛋白 mRNA 表达水平，以及孵化时胸肌中磷酸化肝激酶 B1 和磷酸化 AMPK 的蛋白丰度。总之，肌内注射 CrPyr 可改善肉鸡的能量状态，增加葡萄糖转运蛋白的基因表达，促进胸肌中的糖酵解，这可能与激活的 AMPK 途径有关。
