Zhao M M, Gao T, Zhang L, Li J L, Lv P A, Yu L L, Gao F, Zhou G H
College of Animal Science and Technology, Jiangsu Key Laboratory of Animal Origin Food Production and Safety Guarantee;Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control,Nanjing Agricultural University,Nanjing 210095,China.
Animal. 2017 Oct;11(10):1689-1697. doi: 10.1017/S1751731117000374. Epub 2017 Feb 21.
The effects of in ovo feeding (IOF) of creatine pyruvate (CrPyr) on the hatchability, growth performance and energy status of embryos and broilers (Arbor Acres) were investigated. Five treatments were arranged as non-injected treatment (Control), 0.6 ml physiological saline (0.75%) injected treatment (Saline), and IOF treatments injected with 0.6 ml physiological saline (0.75%) containing 3, 6 or 12 mg CrPyr (CrPyr3, CrPyr6 or CrPyr12) into the amnion per fertile egg on day 17.5 of incubation. After hatching, 80 male chicks from each treatment with similar weight close to the average BW of their pooled group were selected and randomly assigned into eight replicates of 10 chicks each. The results showed that the hatchability was not affected among groups, whereas the hatching weight of broilers in CrPyr12 was significantly higher than the control and saline groups (P0.05). Irrespective of dosage, the concentrations of creatine and phosphocreatine, and activities of creatine kinase in embryos were enhanced in CrPyr treatments at 19 E when compared with the control and saline groups (P<0.05). The activities of glucose-6-phosphatase in liver in CrPyr6 and CrPyr12 treatments were higher than the control and saline groups at 19 E (P<0.05). In conclusion, these results indicated that IOF of CrPyr, especially at the level of 12 mg/egg, could improve energy status of embryos and hatchlings, which was useful for enhancing hatching weight, BW and pectoral muscle weight until the end of the experiments at 21 days post-hatch in broilers.
研究了在鸡胚中注射肌酸丙酮酸（CrPyr）对胚胎和肉鸡（艾维茵肉鸡）孵化率、生长性能及能量状态的影响。设置了5种处理，分别为未注射处理（对照）、注射0.6毫升生理盐水（0.75%）处理（生理盐水组），以及在孵化第17.5天向每个受精蛋的羊膜内注射0.6毫升含3毫克、6毫克或12毫克CrPyr（CrPyr3、CrPyr6或CrPyr12）的生理盐水的鸡胚注射处理。孵化后，从每个处理中选取80只体重相近且接近其合并组平均体重的雄性雏鸡，随机分为8个重复，每个重复10只雏鸡。结果表明，各组间孵化率未受影响，而CrPyr12组肉鸡的出壳重显著高于对照组和生理盐水组（P<0.05）。与对照组和生理盐水组相比，在胚胎发育19日龄时，无论剂量如何，CrPyr处理组胚胎中的肌酸和磷酸肌酸浓度以及肌酸激酶活性均有所提高（P<0.05）。在胚胎发育19日龄时，CrPyr6和CrPyr12处理组肝脏中的葡萄糖-6-磷酸酶活性高于对照组和生理盐水组（P<0.05）。总之，这些结果表明，注射CrPyr，尤其是12毫克/蛋的水平，可改善胚胎和雏鸡的能量状态，这有助于提高出壳重、体重以及胸肌重量，直至肉鸡孵化后21天实验结束。
