Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences, Allahabad, 211007, India.
Nanotechnology Application Centre, Faculty of Science, University of Allahabad, Allahabad, 211002, India.
J Biomed Mater Res A. 2018 Mar;106(3):641-651. doi: 10.1002/jbm.a.36268. Epub 2017 Nov 2.
Diabetic wounds are susceptible to microbial infection. The treatment of these wounds requires a higher payload of growth factors. With this in mind, the strategy for this study was to utilize a novel payload comprising of Eudragit RL/RS 100 nanofibers carrying the bacterial inhibitor gentamicin sulfate (GS) in concert with recombinant human epidermal growth factor (rhEGF); an accelerator of wound healing. GS containing Eudragit was electrospun to yield nanofiber scaffolds, which were further modified by covalent immobilization of rhEGF to their surface. This novel fabricated nanoscaffold was characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The thermal behavior of the nanoscaffold was determined using thermogravimetric analysis and differential scanning calorimetry. In the in vitro antibacterial assays, the nanoscaffolds exhibited comparable antibacterial activity to pure gentemicin powder. In vivo work using female C57/BL6 mice, the nanoscaffolds induced faster wound healing activity in dorsal wounds compared to the control. The paradigm in this study presents a robust in vivo model to enhance the applicability of drug delivery systems in wound healing applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 641-651, 2018.
糖尿病伤口容易受到微生物感染。这些伤口的治疗需要更高的生长因子负载量。考虑到这一点，本研究的策略是利用一种新型的载药系统，该系统由携带细菌抑制剂硫酸庆大霉素（GS）的 Eudragit RL/RS 100 纳米纤维与重组人表皮生长因子（rhEGF）组成；rhEGF 是一种促进伤口愈合的物质。将含有 GS 的 Eudragit 进行静电纺丝以得到纳米纤维支架，然后通过 rhEGF 与支架表面的共价固定化对其进行进一步修饰。使用扫描电子显微镜、傅里叶变换红外光谱和 X 射线衍射对新型纳米支架进行了表征。使用热重分析和差示扫描量热法确定纳米支架的热行为。在体外抗菌试验中，纳米支架表现出与纯庆大霉素粉末相当的抗菌活性。在使用雌性 C57/BL6 小鼠的体内研究中，与对照组相比，纳米支架在背部伤口中诱导更快的伤口愈合活性。本研究中的范例提供了一个强大的体内模型，以增强药物输送系统在伤口愈合应用中的适用性。 © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 641-651, 2018.
