Department of Food Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Department of Leisure, Recreation and Tourism Management, Tzu Hui Institute of Technology, Pingtung, Taiwan.
J Food Drug Anal. 2017 Oct;25(4):854-861. doi: 10.1016/j.jfda.2016.10.015. Epub 2016 Dec 8.
This study aimed to investigate the direct and immune-stimulated antiproliferative activities of jelly fig achenes fractions including pectinesterase inhibitors, crude polyphenols extract, and purified polyphenols extract (PP). Beside the measurement of cell viability of U937, the quantity of cytokines in conditioned medium and morphologic changes in leukemia were observed. After surveying all fractions in jelly fig, the obtained fractions of polyphenol exhibited the highest stimulating effects and directly cytotoxic effects against leukemia with the lowest effect found in protein fractions. The leukemia treated by our PP fraction showed dose-dependent response between the concentration and G2/M cell numbers of the U937 cells. The PP fraction had more pronounced effect on immune-stimulated than direct antiproliferative activities. The finding was also supported by morphological analysis by showing the formation of apoptotic bodies and differentiation from immature U937 cells into mature monocytes/macrophages on cells cultured with PP-conditioned medium. In conclusion, polyphenol fraction of pectinesterase inhibitors from jelly fig showed the immune-stimulated antiproliferative activities against U937 cell.
本研究旨在探究包括果胶酯酶抑制剂、粗多酚提取物和纯化多酚提取物（PP）在内的木奶果种仁各部位的直接和免疫刺激抗增殖活性。除了检测 U937 细胞活力外，还观察了条件培养基中细胞因子的数量和白血病细胞的形态变化。在对木奶果的所有部位进行调查后，发现多酚部位对白血病的刺激作用和直接细胞毒性作用最高，而蛋白质部位的作用最低。用我们的 PP 部位处理的白血病表现出 U937 细胞浓度和 G2/M 细胞数量之间的剂量依赖性反应。PP 部位对免疫刺激的作用比对直接抗增殖作用更为显著。形态学分析也支持这一发现，结果表明，在含有 PP 条件培养基的细胞培养中，形成了凋亡小体，并且未成熟的 U937 细胞向成熟单核细胞/巨噬细胞分化。总之，果胶酯酶抑制剂的多酚部位显示出对 U937 细胞的免疫刺激抗增殖活性。
