State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, Chengdu 610041, China; Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral diseases, West China Hospital of Stomatology, Sichuan University, No. 14, Section 3, Renmin South Road, Chengdu 610041, China; Department of Operative Dentistry and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
J Dent. 2017 Dec;67:88-93. doi: 10.1016/j.jdent.2017.10.001. Epub 2017 Oct 5.
To evaluate the effects of 8% arginine-containing toothpaste on the dental plaque of no caries (NC) and high caries (HC) individuals in situ.
6 no caries (DMFT=0) and 6 high caries (DMFT≥6) individuals wearing a self-developed in situ dental plaque acquisition device were involved in a randomized double-blinded crossover study for 6 weeks: including lead-in (1 week), arginine-free (2 weeks), washout (1 week) and arginine-active (2 weeks) stages. The in situ plaque samples were collected at the endpoint of arginine-free and arginine-active stages and subjected to lactic acid production, metabolic activity, live/dead bacteria ratio and total biofilm biomass detections.
The arginine-containing dentifrice reduced lactic acid production significantly in both the NC and HC groups, while the inhibitory abilities in the HC group were stronger than that in the NC group. In addition, the arginine-containing dentifrice didn't significantly decrease the metabolic activity, live/dead bacteria ratio and total biofilm biomass in either the NC or the HC group.
Arginine-containing toothpaste can significantly reduce the lactic acid production from the in situ plaques to a low level without changing the metabolic activity, live/dead bacteria ratio and total biofilm biomass through a critical clinical randomized double-blinded crossover study.
Arginine is a potential ecological prevention and control agent for dental caries. Meanwhile, the in situ model is an easy and pragmatic way to evaluate oral hygiene products (clinical trial registration: ChiCTR-INR-16010226).
评价含 8%精氨酸牙膏对无龋（NC）和高龋（HC）个体口腔菌斑的影响。
纳入 6 名无龋（DMFT=0）和 6 名高龋（DMFT≥6）个体，使用自行开发的口腔菌斑采集装置进行随机双盲交叉研究 6 周：包括导入期（1 周）、无精氨酸期（2 周）、洗脱期（1 周）和含精氨酸期（2 周）。在无精氨酸期和含精氨酸期结束时采集口腔菌斑样本，进行产酸、代谢活性、活菌/死菌比和总生物膜生物量检测。
含精氨酸牙膏可显著降低 NC 和 HC 两组的菌斑产酸，且 HC 组的抑制作用强于 NC 组。此外，含精氨酸牙膏对 NC 组或 HC 组的代谢活性、活菌/死菌比和总生物膜生物量均无显著降低作用。
通过一项严格的临床随机双盲交叉研究表明，含精氨酸牙膏可显著降低口腔菌斑的产酸，使其降低至低水平，同时不改变代谢活性、活菌/死菌比和总生物膜生物量。
精氨酸是一种有潜力的口腔生态防控剂。同时，原位模型是一种评价口腔卫生用品的简单实用方法（临床试验注册：ChiCTR-INR-16010226）。
