Department of Biology and Biological Engineering, Chalmers University of Technology, SE412 96 Gothenburg, Sweden.
Department of Biology and Biological Engineering, Chalmers University of Technology, SE412 96 Gothenburg, Sweden; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Trends Biotechnol. 2017 Dec;35(12):1156-1168. doi: 10.1016/j.tibtech.2017.08.011. Epub 2017 Oct 4.
Bioprocessing offers a sustainable and green approach to the production of chemicals. However, a bottleneck in introducing bioprocesses is cell factory development, which is costly and time-consuming. A systems biology approach can expedite cell factory design by using genome-wide analyses alongside mathematical modeling to characterize and predict cellular physiology. This approach can drive cycles of design, build, test, and learn implemented by metabolic engineers to optimize the cell factory performance. Streamlining of the design phase requires a clearer understanding of metabolism and its regulation, which can be achieved using quantitative and integrated omic characterization, alongside more advanced analytical methods. We discuss here the current impact of systems biology and challenges of closing the gap between bioprocessing and more traditional methods of chemical production.
生物加工为化学品的生产提供了一种可持续的绿色方法。然而，引入生物加工的一个瓶颈是细胞工厂的开发，这既昂贵又耗时。系统生物学方法可以通过使用全基因组分析和数学建模来对细胞生理学进行特征描述和预测，从而加速细胞工厂的设计。这种方法可以通过代谢工程师来驱动设计、构建、测试和学习的循环，以优化细胞工厂的性能。通过设计阶段的简化需要更清楚地了解代谢及其调控，这可以通过使用定量和综合的组学特征描述以及更先进的分析方法来实现。在这里，我们讨论了系统生物学的当前影响以及缩小生物加工和更传统的化学生产方法之间差距的挑战。
