Culhane Kyle, Jiang Ke, Neumann Aaron, Pinchuk Anatoliy O
Center for Biofrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Pkwy, Colorado Springs, Colorado 80918, United States.
Department of Pathology, University of New Mexico, 915 Camino de Salud, Albuquerque, NM 87131, United States.
MRS Adv. 2017;2(42):2287-2294. doi: 10.1557/adv.2017.98. Epub 2017 Jan 24.
We used a laser-directed fabrication to create silver nanostructures on glass cover slips via photo-reduction. The resulting silver films exhibited plasmonic properties which show promise in application towards surface enhanced Raman spectroscopy (SERS). The enhancement factor calculated for the deposits was approximately ~10 using the standard thiophenol, which is comparable to other SERS-active plasmonic nanostructures fabricated through more complex techniques, such as electron beam lithography. The silver nanostructures were then employed in the enhancement of Raman signals from -butyryl-L-homoserine lactone, a signaling molecule relevant to bacteria quorum sensing. In particular, the work presented here shows that the laser-deposited plasmonic nanostructures are promising candidates for monitoring concentrations of signaling molecules within biofilms containing quorum sensing bacteria.
我们采用激光定向制造技术，通过光还原在玻璃盖玻片上制备银纳米结构。所得银膜表现出等离子体特性，在表面增强拉曼光谱（SERS）应用中显示出前景。使用标准硫酚计算得出沉积物的增强因子约为~10，这与通过更复杂技术（如电子束光刻）制造的其他SERS活性等离子体纳米结构相当。然后将银纳米结构用于增强来自γ-丁酰基-L-高丝氨酸内酯的拉曼信号，γ-丁酰基-L-高丝氨酸内酯是一种与细菌群体感应相关的信号分子。特别是，此处展示的工作表明，激光沉积的等离子体纳米结构有望用于监测含有群体感应细菌的生物膜内信号分子的浓度。
