Light, Nanomaterials and Nanotechnology (L2N), FRE-CNRS 2019, Institute Charles Delaunay (ICD), University of Technology of Troyes, 12 Rue Marie Curie CS 42060, 10004 Troyes CEDEX, France.
Dipartimento di Scienze Agroalimentari, Ambientali e Animali (DI4A), Università degli Studi di Udine, Via Sondrio 2/A, 33100 Udine, Italy.
Biosensors (Basel). 2019 Apr 10;9(2):53. doi: 10.3390/bios9020053.
In this study, stable gold nanoparticles (AuNPs) are fabricated for the first time on commercial ultrafine glass coverslips coated with gold thin layers (2 nm, 4 nm, 6 nm, and 8 nm) at 25 °C and annealed at high temperatures (350 °C, 450 °C, and 550 °C) on a hot plate for different periods of time. Such gold nanostructured coverslips were systematically tested via surface enhanced Raman spectroscopy (SERS) to identify their spectral performances in the presence of different concentrations of a model molecule, namely 1,2-bis-(4-pyridyl)-ethene (BPE). By using these SERS platforms, it is possible to detect BPE traces (10 M) in aqueous solutions in 120 s. The stability of SERS spectra over five weeks of thiol-DNA probe (2 µL) deposited on gold nano-structured coverslip is also reported.
本研究首次在商业超净玻璃载玻片上制备了稳定的金纳米颗粒（AuNPs），这些载玻片上涂有 2nm、4nm、6nm 和 8nm 厚的金薄层，然后在热板上于高温（350°C、450°C 和 550°C）下退火不同时间。通过表面增强拉曼光谱（SERS）对这些金纳米结构载玻片进行了系统测试，以确定它们在存在不同浓度的模型分子（即 1,2-双（4-吡啶基）乙烯（BPE））时的光谱性能。使用这些 SERS 平台，有可能在 120 秒内检测到水溶液中的 BPE 痕量（10μM）。还报告了在金纳米结构载玻片上沉积 2µL 巯基-DNA 探针后，SERS 光谱在五周内的稳定性。
