Han Shuyu, Ma Chaoxiong, Hu Minghua, Wang Yuanyuan, Ma Fangli, Tao Ning, Qin Zhihai
Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
China Pharmaceutical University, Nanjing, Jiangsu, China.
Cell Biochem Funct. 2017 Oct;35(7):414-419. doi: 10.1002/cbf.3290. Epub 2017 Oct 8.
Reversing the function of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Here, we isolated a novel polysaccharide from Dictyophora indusiata (ZSP4) and examined its effects on the function of prostate CAFs. The supernatant of prostate CAFs can stimulate the proliferation of immune cells and inhibit the growth of CD4+/CD8+ T cells. However, after ZSP4 stimulation, the functions of prostate CAFs were inhibited. The mechanism experiment shows that ZSP4 can stimulate prostate CAFs by down-regulating the expression of α-smooth muscle actin. Polysaccharides extracted from Dictyophora indusiata stimulate the proliferation of immune cells and reverse the immune-suppressive functions of prostate CAFs, shedding new light on the development of novel anticancer strategies. The endocrine therapy used to treat prostate cancer aims to eliminate androgenic activity from prostatic tissue; these therapies are painful and of poor therapeutic effect. In this study, we found that polysaccharides extracted from Dictyophora indusiata may affect the micro-environment of tumours and inhibit the growth of the tumours. Our results suggest that polysaccharides may modulate negative immune regulation and enhance antitumour immunity, which is important for clinical therapy.
逆转癌症相关成纤维细胞（CAFs）的功能可能会提高癌症治疗的疗效。在此，我们从竹荪中分离出一种新型多糖（ZSP4），并研究了其对前列腺CAFs功能的影响。前列腺CAFs的上清液可刺激免疫细胞增殖并抑制CD4+/CD8+ T细胞的生长。然而，在ZSP4刺激后，前列腺CAFs的功能受到抑制。机制实验表明，ZSP4可通过下调α-平滑肌肌动蛋白的表达来刺激前列腺CAFs。从竹荪中提取的多糖可刺激免疫细胞增殖并逆转前列腺CAFs的免疫抑制功能，为新型抗癌策略的开发提供了新线索。用于治疗前列腺癌的内分泌疗法旨在消除前列腺组织中的雄激素活性；这些疗法痛苦且治疗效果不佳。在本研究中，我们发现从竹荪中提取的多糖可能会影响肿瘤的微环境并抑制肿瘤生长。我们的结果表明，多糖可能调节负性免疫调节并增强抗肿瘤免疫力，这对临床治疗具有重要意义。
