Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
Int J Biol Macromol. 2013 Oct;61:453-8. doi: 10.1016/j.ijbiomac.2013.08.007. Epub 2013 Aug 22.
A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3 M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation-renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti-tumor activity of this polysaccharide after denaturation-renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti-tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti-tumor activity than that of PD3, suggesting that the denaturation-renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata.
从裂褶菌中分离得到一种三螺旋多糖（PD3）。在 0.3M 稀 NaOH 溶液中变性并通过顺序透析复性后，得到再生多糖（RPD3）。RPD3 的物理化学性质，包括特性黏度 [η]、分子量（Mw）和旋光度与 PD3 相似，这表明 RPD3 在变性-复性后也具有三螺旋结构。然而，PD3 和 RPD3 的特性黏度对 NaOH 溶液浓度的依赖性不同，这表明 RPD3 的链紧密程度低于 PD3。进一步研究了这种多糖经变性-复性处理后的抗肿瘤活性。PD3 和 RPD3 均未显示出对 S-180 细胞的直接细胞毒性，但在体内表现出抗肿瘤活性。同时，高剂量组的 RPD3 表现出比 PD3 更高的抗肿瘤活性，表明变性-复性处理提高了裂褶菌多糖的生物活性。
