School of Public Health, Guizhou Medical University, Guiyang 550025, China.
Key Laboratory of Environmental Pollution Monitoring Control Ministry of Education, Guizhou Medical University, Guiyang 550025, China.
Molecules. 2022 Apr 28;27(9):2806. doi: 10.3390/molecules27092806.
Arsenic (As) is common in the human living environment and a certain amount of exposure to As can lead to liver damage; this toxic effect has been proved to be closely related to intracellular PINK1/Parkin pathway-mediated mitophagy. Dictyophora is an edible fungus that extracts polysaccharides with antioxidant and hepatoprotective effects. In the present study, we demonstrated that As induced the onset of mitophagy in hepatocytes by stimulating cellular production of ROS to activate PINK1/Parkin, and the extent of damage increased with increased As-induced toxicity. Dictyophora polysaccharide (DIP) has the ability to scavenge intracellular ROS, which can inhibit oxidative stress injury and inhibit the PINK/Parkin pathway through its receptors or efficacious proteins, thus preventing mitochondrial autophagy and alleviating the hepatotoxicity of As. In conclusion, our results indicate that DIP can reduce As-induced PINK1/Parkin pathway-mediated hepatic mitophagy through scavenging ROS and exert hepatoprotective effects, providing experimental data and theoretical basis for the development of medicinal value of Dictyophora as a dual-use food and medicinal fungus.
砷（As）在人类生活环境中很常见，一定量的暴露于 As 会导致肝脏损伤；这种毒性作用已被证明与细胞内 PINK1/Parkin 通路介导的线粒体自噬密切相关。香菇是一种食用真菌，可提取具有抗氧化和保肝作用的多糖。在本研究中，我们证明了 As 通过刺激细胞产生 ROS 来激活 PINK1/Parkin，从而诱导肝细胞发生线粒体自噬，并且随着 As 诱导的毒性增加，损伤程度增加。香菇多糖（DIP）具有清除细胞内 ROS 的能力，可通过其受体或有效蛋白抑制氧化应激损伤和抑制 PINK/Parkin 通路，从而防止线粒体自噬并减轻 As 的肝毒性。总之，我们的结果表明，DIP 通过清除 ROS 可减少 As 诱导的 PINK1/Parkin 通路介导的肝线粒体自噬，发挥保肝作用，为香菇作为两用食品和药用真菌的药用价值开发提供了实验数据和理论依据。
