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用阿萨姆种茶树种子优化皂苷提取条件及其在天然洗涤剂中的应用

Optimisation of saponin extraction conditions with Camellia sinensis var. assamica seed and its application for a natural detergent.

作者信息

Gong Wanying, Huang Yewei, Ji Aibing, Peng Wenshu, Liu Cong, Zeng Yin, Yang Ruijuan, Yan Liang, Wang Xuanjun, Sheng Jun

机构信息

Key Laboratory of Pu-er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, China.

Pu'er Institute of Pu-erh Tea, Pu-er, China.

出版信息

J Sci Food Agric. 2018 Apr;98(6):2312-2319. doi: 10.1002/jsfa.8721. Epub 2017 Dec 11.

DOI:10.1002/jsfa.8721
PMID:28990656
Abstract

BACKGROUND

Camellia sinensis var. assamica seed cake (a by-product of tea-seed oil) is an abundant resource with poor utilisation. C. sinensis var. assamica seed saponin (CSS) is one kind of non-ionic surfactant. In this study, the CSS extraction conditions were optimised by response surface methodology (RSM) and then the CSS detergent was developed. Additionally, the safety and decontamination ability of the developed detergent were evaluated.

RESULTS

The optimised extraction conditions were including the extracting temperature of 40.04 °C, extraction time of 4.97 h, ethanol concentration of 64.11% and liquid-solid ratio of 14.57:1 mL g . The formula of the CSS detergent was as follows: 20% crude CSS, 0.3% oxidised tea polyphenols (OTPs), 0.2% nisin, 0.3% sodium dehydroacetate, 0.7% sodium alginate and 0.5% sodium polyacrylate. The LD of the CSS detergent exceeds 14 g kg in mice, indicating the detergent was non-toxic. Both of the emulsifying and the pesticide residues removal abilities of the CSS detergent were significantly stronger than the commercial detergent.

CONCLUSION

A natural tea seed saponin detergent with good safety and decontamination ability was successfully developed. This can make better use of the tea seed cake, thereby creating added value in the tea seed oil industry. © 2017 Society of Chemical Industry.

摘要

背景

阿萨姆种茶树籽饼(茶籽油的一种副产品)是一种资源丰富但利用率低的物质。阿萨姆种茶树籽皂苷(CSS)是一种非离子表面活性剂。在本研究中,通过响应面法(RSM)优化了CSS的提取条件,然后开发了CSS洗涤剂。此外,还评估了所开发洗涤剂的安全性和去污能力。

结果

优化后的提取条件为提取温度40.04℃、提取时间4.97小时、乙醇浓度64.11%、液固比14.57:1 mL/g。CSS洗涤剂的配方如下:20%粗CSS、0.3%氧化茶多酚(OTP)、0.2%乳酸链球菌素、0.3%脱氢乙酸钠、0.7%海藻酸钠和0.5%聚丙烯酸钠。CSS洗涤剂在小鼠中的半数致死量超过14 g/kg,表明该洗涤剂无毒。CSS洗涤剂的乳化能力和去除农药残留的能力均明显强于市售洗涤剂。

结论

成功开发出一种具有良好安全性和去污能力的天然茶籽皂苷洗涤剂。这可以更好地利用茶籽饼,从而在茶籽油行业创造附加值。©2017化学工业协会。

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