University of the Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
J Nat Prod. 2017 Oct 27;80(10):2615-2619. doi: 10.1021/acs.jnatprod.7b00056. Epub 2017 Oct 9.
Our natural products discovery program utilizes endophytic actinomycetes associated with plants and employs biological assays and HPLC-based metabolite profiles as the preliminary screen to identify strains of interest, followed by large-scale fermentation and isolation, leading to new and/or bioactive natural products. Six new trialkyl-substituted aromatic acids, namely, lorneic acids E-J (1-6), together with two known analogues (7 and 8), were isolated and identified from the culture extract of Streptomyces sp. KIB-H1289, an endophytic actinomycete obtained from the inner tissue of the bark of Betula mandshurica Nakai. The structures were characterized by interpretation of their spectroscopic data, mainly 1D and 2D NMR. Among them, compound 5 contains a unique disulfide bond that is presumably derived from N-acetylcysteine. All isolated metabolites were evaluated for their inhibitory activity on tyrosinase.
我们的天然产物发现计划利用与植物相关的内生放线菌，并采用生物测定和基于 HPLC 的代谢物图谱作为初步筛选，以鉴定有价值的菌株，然后进行大规模发酵和分离，从而获得新的和/或具有生物活性的天然产物。从内生放线菌 Streptomyces sp. KIB-H1289 的培养提取物中分离并鉴定了 6 种新的三烷基取代芳香酸，即 lor-neic 酸 E-J（1-6），以及两种已知类似物（7 和 8）。这些结构是通过解释它们的光谱数据，主要是 1D 和 2D NMR 来确定的。其中，化合物 5 含有一个独特的二硫键，推测它来自 N-乙酰半胱氨酸。所有分离出的代谢物都评估了它们对酪氨酸酶抑制活性。
