Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, P. R. China.
Livestock and Poultry Healthy Breeding Research Sub-center of Biochip Beijing Engineering Research Center, Department of Veterinary Medicine, Beijing University of Agriculture, Beijing, P. R. China.
J Sep Sci. 2022 Apr;45(7):1317-1325. doi: 10.1002/jssc.202100829. Epub 2022 Feb 25.
Codonopsis radix is an important edible and medicinal plant resource for immunomodulation in China and Southeast Asia. However, the chemical quality evaluation of C. radix in Chinese Pharmacopoeia (2020 Version) is still lacking; therefore, it is necessary to develop an effective method to evaluate its quality accurately and systematically. Herein, a reliable method for a comprehensive chemical analysis of bioactive compounds in C. radix by ultra-performance liquid chromatography-diode array detector was developed based on the quality marker (Q-marker) concept, which can efficiently reflect its immune activity. Our previous research explored the seven potential bioactive compounds reflecting the immune regulation activities of C. radix by spectrum-effect relationship analysis. Therefore, in this study, we researched on establishing a quality control method and selected the modern pharmacodynamic experiment of immune regulation to verify the potential bioactive compounds as quality markers. A real quality control method that reflected the traditional efficacy of C. radix in strengthening the spleen and tonifying lungs was developed. Furthermore, the C. radix extract and the seven bioactive compounds could promote the proliferation of immune-related cells and regulate the secretion of inflammatory factors, thus playing a role in immune regulation.
党参是中国和东南亚地区具有免疫调节作用的重要食用和药用植物资源。然而，《中国药典》（2020 年版）中对党参的化学质量评价仍存在不足；因此，有必要开发一种准确、系统的有效方法来评价其质量。本研究基于质量标志物（Q-marker）的概念，建立了党参中生物活性化合物的超高效液相色谱-二极管阵列检测的全面化学分析可靠方法，该方法能够有效地反映其免疫活性。我们之前的研究通过光谱-效应关系分析探讨了反映党参免疫调节活性的七种潜在生物活性化合物。因此，本研究建立了一种质量控制方法，并选择现代免疫调节药效学实验对潜在生物活性化合物作为质量标志物进行验证。建立了反映党参健脾益肺传统功效的真正质量控制方法。此外，党参提取物和七种生物活性化合物可以促进免疫相关细胞的增殖，并调节炎症因子的分泌，从而发挥免疫调节作用。
