Ogawa Taiji, Honda-Ogawa Mariko, Ikebe Kazunori, Notomi Yumiko, Iwamoto Yoshiko, Shirobayashi Itsuki, Hata Seiko, Kibi Masahito, Masayasu Shizuko, Sasaki Satoshi, Kawabata Shigetada, Maeda Yoshinobu
Department of Prosthodontics, Gerodontology and Oral Rehabilitation, Osaka University Graduate School of Dentistry.
Department of Oral and Molecular Microbiology, Osaka University Graduate School of Dentistry.
J Oral Sci. 2017 Dec 27;59(4):549-555. doi: 10.2334/josnusd.16-0722. Epub 2017 Oct 6.
Over 700 bacterial species have been detected in the oral cavity. Several studies have suggested that periodontitis is associated with systemic disorders such as diabetes mellitus, indicating a key role for oral microbiota in human health. However, the relationship between oral microbiota and diabetes has not been well clarified. Therefore, we conducted microbiome analysis of saliva samples obtained from 15 elderly residents (3 with type 2 diabetes mellitus [DM] and 12 without diabetes [non-DM]) at three different nursing homes, as well as 9 young healthy controls (HC). Genomic DNA was extracted from each sample, and then the V4 region of the 16S rRNA gene was amplified and sequenced. Alpha diversity, in terms of operational taxonomic unit richness, was significantly higher in samples from the non-DM group than in those from the HC group. Weighted UniFrac distance analysis showed that salivary microbial communities in the DM group were separately clustered. Furthermore, in the DM group, Actinomyces and Selenomonas showed significantly higher abundance, whereas Alloprevotella showed significantly lower abundance, relative to the non-DM group. Although our findings were limited by the small sample size, oral bacterial diversity in the DM group was clearly different from that in the non-DM group.
在口腔中已检测到700多种细菌。多项研究表明，牙周炎与糖尿病等全身性疾病有关，这表明口腔微生物群在人类健康中起着关键作用。然而，口腔微生物群与糖尿病之间的关系尚未得到充分阐明。因此，我们对从三个不同养老院的15名老年居民（3名2型糖尿病患者[DM]和12名非糖尿病患者[非DM]）以及9名年轻健康对照者（HC）获取的唾液样本进行了微生物组分析。从每个样本中提取基因组DNA，然后对16S rRNA基因的V4区域进行扩增和测序。就操作分类单元丰富度而言，非DM组样本的α多样性显著高于HC组样本。加权UniFrac距离分析表明，DM组的唾液微生物群落单独聚类。此外，与非DM组相比，DM组中放线菌属和月形单胞菌属的丰度显著更高，而别普雷沃菌属的丰度显著更低。尽管我们的研究结果受样本量小的限制，但DM组的口腔细菌多样性明显不同于非DM组。
