Chaizhunusova Nailya, Madiyeva Madina, Tanaka Kimio, Hoshi Masaharu, Kawano Noriyuki, Noso Yoshihiro, Takeichi Nobuo, Rakhypbekov Tolebay, Urazalina Nailya, Dovgal Galina, Rymbaeva Tamara, Tokanova Sholpan, Beisengazina Meruert, Kembayeva Kulypash, Inoue Ken
Department of Nutrition and Hygienic Disciplines, Semey State Medical University, Abay st. 103, Semey, 071400, Kazakhstan.
Department of Oncology and Visual Diagnostic, Semey State Medical University, Abay st. 103, Semey, 071400, Kazakhstan.
Radiat Environ Biophys. 2017 Nov;56(4):337-343. doi: 10.1007/s00411-017-0717-2. Epub 2017 Oct 9.
More than 400 nuclear explosion tests were conducted at the Semipalatinsk Nuclear Test Site (SNTS) and significant radioactive substances were released. The long-term consequences of the activities at the SNTS and the appearance of any hereditary effects remain insufficiently studied about 25 years after the test site was closed. The population living in villages near the SNTS are considered to have been heavily exposed to external and internal radiation. This study aims to perform an assessment and comprehensive cytogenetic analysis of the inhabitants living near the SNTS, and their first-(F1) and second-(F2) generation children. Residents of the East Kazakhstan region living in the area covered by the former SNTS were included in the study. To evaluate the hereditary effects of nuclear testing, comprehensive chromosome analyses were performed in lymphocytes using conventional Giemsa and fluorescent in situ hybridization methods in 115 F1 and F2 descendants in the villages of Dolon and Sarzhal, which were heavily contaminated. The parents of the subjects had permanently lived in the villages. A higher number of stable-type chromosome aberrations such as translocations was found in these residents than in 80 residents of the control area, Kokpecty, which indicates the possibility that radiation had biological effects on the exposed subjects.
在塞米巴拉金斯克核试验场进行了400多次核爆炸试验，释放了大量放射性物质。在试验场关闭约25年后，塞米巴拉金斯克核试验场活动的长期后果以及任何遗传效应的出现仍未得到充分研究。生活在塞米巴拉金斯克核试验场附近村庄的居民被认为受到了大量内外辐射照射。本研究旨在对生活在塞米巴拉金斯克核试验场附近的居民及其第一代（F1）和第二代（F2）子女进行评估和全面的细胞遗传学分析。居住在前塞米巴拉金斯克核试验场覆盖地区的东哈萨克斯坦地区居民被纳入研究。为了评估核试验的遗传效应，采用常规吉姆萨染色法和荧光原位杂交法，对受严重污染的多隆村和萨尔扎尔村的115名F1和F2后代的淋巴细胞进行了全面的染色体分析。研究对象的父母一直居住在这些村庄。与对照地区科克佩蒂的80名居民相比，这些居民中发现了更多的稳定型染色体畸变，如易位，这表明辐射可能对受照射对象产生了生物学效应。
