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[从玄参中分离内生真菌的鉴定及系统发育分析]

[Identification and phylogenetic analysis of endophytic fungi isolated from Scrophularia ningpoensis].

作者信息

Wu Xiao-Li, Song Xu-Hong, Yang Hong-Guo, Liu Fei, Zhang Xue, Liu Jin-Liang, Chen Da-Xia, Li Long-Yun

机构信息

Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.

Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica, Chongqing 400065, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2017 Mar;42(5):902-911. doi: 10.19540/j.cnki.cjcmm.20170217.010.

Abstract

The endophytic fungi from root, main stem, branch and leaf of Scrophularia ningpoensis were isolated and identified from Wulong and Chongqing, and the population diversity analysis and phylogenetic analysis were followed. The result indicated that, as to population diversity index, S. ningpoensis from Wulong: leaf>main stem=branch>root, branch from Chongqing>branch from Wulong. Fifty-eight endophytic fungi were obtained, most of which were the pathogens of the plant. Colletotrichum was the prevailing genus, of which C. gloeosporioides and C. boninense were the prevailing strains. Leaf and seedlings might be the main path of infection. Endophytic fungi and pathogen might convert to each other, influenced by such factors as environment, genotype et al.

摘要

从重庆武隆的玄参根、主茎、枝条和叶片中分离并鉴定内生真菌,随后进行种群多样性分析和系统发育分析。结果表明,就种群多样性指数而言,武隆玄参:叶>主茎 = 枝条>根,重庆枝条>武隆枝条。共获得58株内生真菌,其中多数为植物病原菌。炭疽菌属为优势属,其中胶孢炭疽菌和香蕉炭疽菌为优势菌株。叶片和幼苗可能是主要侵染途径。内生真菌与病原菌可能受环境、基因型等因素影响而相互转化。

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