Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering and ‡School of Resource and Environment, University of Jinan , Jinan 250022, China.
ACS Appl Mater Interfaces. 2017 Nov 1;9(43):37637-37644. doi: 10.1021/acsami.7b13416. Epub 2017 Oct 18.
The accurate tumor marker detection at an early stage can prevent people from getting cancer to a great extent. Herein, a novel tri-antibody dual-channel biosensing strategy is applied in multianalysis of carcino-embryonic antigen (CEA) and nuclear matrix protein 22 (NMP22). In this immunosensor fabrication process, graphene oxide/polyaniline nanostructures are used as matrix and mesoporous NKF-5-3 is used as labels. Two kinds of antigens can be obtained from the signals of neutral red and toluidine blue, respectively, which are modified on the labels. In this tri-antibody dual-channel biosensing platform, sulfur-doped graphene sheet is synthesized by click chemistry as the framework structure. Majority of the incubations are conducted in individual steps, which ensure the surface incubation more tightly. The detection limit of NMP22 and CEA are 25 and 30 fg/mL, respectively. The low detection limit and excellent stability can ascribe to the tri-antibody dual-channel strategy, which makes the sensor platform from surface to the space. The clinical urine sample analysis achieves a good performance. The urine-based test can avoid the secondary injury on hemophilia or ischemic patients, displaying a potential application in clinical diagnosis.
早期准确的肿瘤标志物检测在很大程度上可以预防癌症。在此，我们应用一种新型的三抗体双通道生物传感策略来进行癌胚抗原（CEA）和核基质蛋白 22（NMP22）的多分析。在这个免疫传感器的制造过程中，氧化石墨烯/聚苯胺纳米结构被用作基质，介孔 NKF-5-3 被用作标记物。两种抗原可以分别从标记物上修饰的中性红和甲苯胺蓝的信号中获得。在这个三抗体双通道生物传感平台中，通过点击化学合成了掺硫石墨烯片作为框架结构。大多数孵育都是在单个步骤中进行的，这确保了表面孵育更加紧密。NMP22 和 CEA 的检测限分别为 25 和 30 fg/mL。低检测限和出色的稳定性归因于三抗体双通道策略，它使传感器平台从表面到空间。对临床尿液样本的分析也取得了良好的效果。基于尿液的测试可以避免对血友病或缺血性患者的二次伤害，在临床诊断中有很好的应用潜力。
