Koh G L, Tucker I G
Department of Pharmacy, University of Queensland, St. Lucia, Australia.
J Pharm Pharmacol. 1988 May;40(5):309-12. doi: 10.1111/j.2042-7158.1988.tb05257.x.
The complex coacervation of sodium carboxymethylcellulose (SCMC) and gelatin has been characterized by chemical analyses of the coacervate and equilibrium fluid phases. The phenol-sulphuric acid (for SCMC) and Lowry (for gelatin) assays were used. Chemically analysed coacervate yield was used to predict optimum coacervation conditions, which occurred at a SCMC-gelatin mixing ratio of 3:7 at pH 3.5. The effects of pH, colloid mixing ratio and total colloid concentration on coacervate yield and composition were studied. The colloid mixing ratio, at which the peak coacervate yields occurred varied with coacervation pH. Increase in the total colloid concentration suppressed coacervation, resulting in a coacervate of higher water content. A similar coacervation mechanism was seen for two viscosity grades SCMC. However, because of the different degree of substitution of these two grades the SCMC-gelatin coacervates had different SCMC contents.
通过对凝聚层和平衡流体相进行化学分析，对羧甲基纤维素钠（SCMC）与明胶的复合凝聚进行了表征。采用了苯酚 - 硫酸法（用于测定SCMC）和洛瑞法（用于测定明胶）。通过化学分析得到的凝聚层产率用于预测最佳凝聚条件，最佳条件出现在pH值为3.5时，SCMC与明胶的混合比例为3:7。研究了pH值、胶体混合比例和总胶体浓度对凝聚层产率和组成的影响。凝聚层产率峰值出现时的胶体混合比例随凝聚pH值而变化。总胶体浓度的增加会抑制凝聚，导致形成含水量更高的凝聚层。两种粘度等级的SCMC表现出相似的凝聚机制。然而，由于这两个等级的取代度不同，SCMC - 明胶凝聚层的SCMC含量也不同。
