Ng M L, Lau L C
Department of Microbiology, National University of Singapore.
Arch Virol. 1988;100(3-4):199-211. doi: 10.1007/BF01487683.
The results obtained from electron microscopy, adsorbed and internalised virus assays and immunofluorescence studies supported that the most likely mode of entry of Kunjin virus into Vero cells was by receptor-mediated endocytosis. This was deduced indirectly from the time sequence of events that occurred. Electron microscopy revealed that endocytosis of the virus through coated vesicles had occurred. The adsorbed and internalised virus assay and immunofluorescence studies showed that there were two factors being recycled during endocytosis: the receptor for the virus and clathrin, the protein found on coated pits and vesicles. The study showed that clathrin was recycled first, followed by the receptor.
电子显微镜、病毒吸附与内化检测以及免疫荧光研究所得结果支持，库京病毒进入Vero细胞最可能的方式是通过受体介导的内吞作用。这是从所发生事件的时间顺序间接推断出来的。电子显微镜显示，病毒通过有被小泡发生了内吞作用。病毒吸附与内化检测以及免疫荧光研究表明，在内吞作用过程中有两个因子被循环利用：病毒受体和网格蛋白，后者是在有被小窝和有被小泡上发现的蛋白质。研究表明，网格蛋白先被循环利用，随后是受体。
