Dardmeh Fereshteh, Alipour Hiva, Gazerani Parisa, van der Horst Gerhard, Brandsborg Erik, Nielsen Hans Ingolf
Biomedicine Group, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
SMI®, Department of Health Science and Technology, Faculty of Medicine, Aalborg University, Aalborg, Denmark.
PLoS One. 2017 Oct 10;12(10):e0185964. doi: 10.1371/journal.pone.0185964. eCollection 2017.
Probiotics have been proposed as alternatives to pharmacological products in several medical conditions including the modulation of obesity, which is frequently associated with poor semen quality. However, effects of probiotics on male fertility have been less investigated. This study assessed the effect of Lactobacillus rhamnosus PB01 (DSM-14870) on sperm kinematic parameters in Normal-weight (NW) and diet-induced obese (DIO) models. NW and DIO C57BL/6NTac mice were divided into two subgroups with or without a single daily dose (1x109CFU) of L. rhamnosus for four weeks. Sperm motility and kinematics together with blood lipid profiles and reproductive hormone levels were assessed using the sperm class analyzer system. Probiotic supplementation increased serum testosterone, LH and FSH levels in both NW and DIO groups resulting in significantly (P<0.05) higher velocity (VSL, VCL and VAP) and percentages of progressively motile sperm and significantly lower percentages of immotile sperm. Other kinematic parameters (Lin, STR, ALH and BCF) were also increased in both probiotic supplemented DIO and NW groups at the 10% level of significance. Probiotic supplemented DIO mice demonstrated significantly higher percentages of progressively motile sperm versus DIO controls. This study demonstrated the potential of L. rhamnosus PB01 as a regulatory agent with positive effects on weight loss and reproductive-hormones, significantly improving sperm motility and kinematic parameters in male DIO models.
益生菌已被提议作为多种医学病症中药理产品的替代品，包括调节肥胖，而肥胖常与精液质量差有关。然而，益生菌对男性生育能力的影响研究较少。本研究评估了鼠李糖乳杆菌PB01（DSM-14870）对正常体重（NW）和饮食诱导肥胖（DIO）模型中精子运动学参数的影响。将NW和DIO C57BL/6NTac小鼠分为两个亚组，一组每天单剂量（1x109CFU）给予鼠李糖乳杆菌，另一组不给予，持续四周。使用精子分类分析仪系统评估精子活力和运动学以及血脂谱和生殖激素水平。补充益生菌使NW组和DIO组的血清睾酮、促黄体生成素（LH）和促卵泡生成素（FSH）水平升高，导致精子速度（直线速度、曲线速度和平均路径速度）显著提高（P<0.05），进行性运动精子百分比显著增加，而不动精子百分比显著降低。在补充益生菌的DIO组和NW组中，其他运动学参数（线性度、直线性、平均侧摆幅度和鞭打频率）在10%的显著性水平上也有所增加。补充益生菌的DIO小鼠与DIO对照组相比，进行性运动精子百分比显著更高。本研究证明了鼠李糖乳杆菌PB01作为一种调节剂的潜力，对体重减轻和生殖激素具有积极影响，能显著改善雄性DIO模型中的精子活力和运动学参数。
