Sanchez-Rodriguez Ana, Idrovo Ingrid I D, Villafranca Rocío, Latorre Nerea, Rielo Juan Antonio, Laburu Ane, Nieto-Román Sandra, Heredia Daniel, González Rubén, García-Cañas Virginia, Laxalde Diego, Simó Carolina, Vieites David R, Roldan Eduardo R S
Department of Biodiversity and Evolutionary Biology, Museo Nacional de Ciencias Naturales (CSIC), Calle José Gutierrez Abascal 2, 28006, Madrid, Spain.
Department of Biogeography and Global Change, Museo Nacional de Ciencias Naturales (CSIC), Calle José Gutierrez Abascal 2, 28006, Madrid, Spain.
Probiotics Antimicrob Proteins. 2024 Oct 23. doi: 10.1007/s12602-024-10388-z.
The administration of probiotics for the treatment of different diseases has gained interest in recent years. However, few studies have evaluated their effects on reproductive traits. The objective of this study was to examine the effect of two mixtures of probiotics, a commercial probiotic (Vivomixx®) and a mix of Lacticaseibacillus rhamnosus GG and Faecalibacterium duncaniae A2-165, on sperm quality in a mouse model. Adult male mice (8 months old) were used for two experimental and one control groups (n = 5 each). The probiotics or physiological serum (control) was administered orally, twice a week, during 5 weeks. Sperm were collected from the cauda epididymis, and their total number, motility, kinematics, morphology, and acrosome integrity were assessed in recently collected samples and after a 60-min in vitro incubation. Results showed a higher percentage of normal sperm in both experimental groups, with fewer head abnormalities than in the control. Differences were found among groups in the morphometry of sperm heads, being more elongated in mice treated with probiotics. Sperm from probiotic-treated mice showed similar total motility when compared to the controls, although the proportion of progressively moving sperm and their vigor of motility were lower. Sperm swimming descriptors were measured with a CASA system. Velocity parameters were similar among groups whereas linearity was higher in mice treated with the commercial probiotic. These results suggest that the administration of probiotics may increase the proportion of sperm with normal morphology and lead to modifications in sperm head shape that may enhance sperm swimming. Studies using a longer administration period would be useful in further characterizing the effect of these probiotic mixtures on sperm quality and fertilization capacity.
近年来，使用益生菌治疗不同疾病受到了关注。然而，很少有研究评估它们对生殖性状的影响。本研究的目的是在小鼠模型中检测两种益生菌混合物、一种商业益生菌（Vivomixx®）以及鼠李糖乳杆菌GG和邓肯粪杆菌A2-165的混合物对精子质量的影响。成年雄性小鼠（8个月大）被用于两个实验组和一个对照组（每组n = 5）。益生菌或生理血清（对照）每周口服给药两次，持续5周。从附睾尾部收集精子，并在最近收集的样本以及体外孵育60分钟后评估其总数、活力、运动学、形态和顶体完整性。结果显示，两个实验组的正常精子百分比均较高，头部异常比对照组少。在精子头部的形态测量方面，各实验组之间存在差异，用益生菌处理的小鼠精子头部更细长。与对照组相比，用益生菌处理的小鼠精子的总活力相似，尽管渐进性运动精子的比例及其运动活力较低。用CASA系统测量精子游动指标。各实验组的速度参数相似，而使用商业益生菌处理的小鼠的线性度较高。这些结果表明，施用益生菌可能会增加形态正常的精子比例，并导致精子头部形状发生改变，这可能会增强精子游动能力。使用更长给药期的研究将有助于进一步确定这些益生菌混合物对精子质量和受精能力的影响。
