Pedretti Stephanie Luzia da Costa, Rena Cícero de Lima, Castãnon Maria Christina Marques Nogueira, Duque Ana Paula do Nascimento, Pereira Fernando Henrique, Nunes Tarcizo Afonso
- Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
- Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
Rev Col Bras Cir. 2017 Sep-Oct;44(5):457-464. doi: 10.1590/0100-69912017005006.
to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process.
were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis.
the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment.
dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.
验证二甲基亚砜和己酮可可碱对大鼠皮瓣活力及组织修复过程的影响。
研究30只Wistar大鼠，制作尾基部宽2cm、长8cm的背部皮瓣。将动物分为三组：对照组（n = 10），在皮瓣床应用用0.9%生理盐水浸湿的纱布30秒；二甲基亚砜组（n = 10），在皮瓣段过渡处给予1ml 5%二甲基亚砜，分五次注射，每次0.2ml；己酮可可碱组（n = 10），给予己酮可可碱20mg/kg，稀释至1ml，在皮瓣段过渡处分五次注射，每次0.2ml。药物在术中单次皮下注射。观察皮瓣颜色和质地变化。术后第10天，检查存活和坏死组织的尺寸，然后切除标本进行组织学分析。
各组间存活和坏死组织长度测量无差异。组织学分析显示，二甲基亚砜组出现新生血管形成、白细胞炎性浸润和结构更致密的结膜基质。己酮可可碱组显示新生血管形成和炎性浸润，伴有中度至重度肉芽组织。对照组远端段坏死率更高。
二甲基亚砜和己酮可可碱影响皮瓣活力和组织修复过程。然而，它们在宏观上并未预防坏死。
