Keelor Joel D, Zambrzycki Stephen, Li Anyin, Clowers Brian H, Fernández Facundo M
School of Chemistry and Biochemistry, Georgia Institute of Technology , Atlanta, Georgia 30332, United States.
Department of Chemistry, Washington State University , Pullman, Washington 99164, United States.
Anal Chem. 2017 Nov 7;89(21):11301-11309. doi: 10.1021/acs.analchem.7b01866. Epub 2017 Oct 27.
Atmospheric pressure drift tube ion mobility spectrometry (AP-DTIMS) was coupled with Fourier transform Orbitrap mass spectrometry. The performance capabilities of this versatile new arrangement were demonstrated for different DTIMS ion gating operation modes and Orbitrap mass spectrometer parameters with regard to sensitivity and resolving power. Showcasing the optimized AP-DTIMS-Orbitrap MS system, isobaric peptide and sugar isomers were successfully resolved and the identities of separated species validated by high-energy collision dissociation experiments.
常压漂移管离子迁移谱（AP-DTIMS）与傅里叶变换轨道阱质谱联用。针对不同的DTIMS离子门控操作模式和轨道阱质谱仪参数，在灵敏度和分辨率方面展示了这种多功能新组合的性能。展示优化后的AP-DTIMS-轨道阱质谱系统，成功分离了等压肽和糖异构体，并通过高能碰撞解离实验验证了分离物种的身份。
