牛组织感染痤疮丙酸杆菌后椎间盘内丙酸含量的定量分析。
Quantification of Propionic Acid in the Bovine Spinal Disk After Infection of the Tissue With Propionibacteria acnes Bacteria.
机构信息
Department of Radiology, University of California at San Francisco, San Francisco, CA.
Department of Orthopedic Surgery, University of California San Francisco, San Francisco, CA.
出版信息
Spine (Phila Pa 1976). 2018 Jun 1;43(11):E634-E638. doi: 10.1097/BRS.0000000000002448.
STUDY DESIGN
Research.
OBJECTIVE
The goal of this study was to investigate whether Propionibacteria acnes infection of the intervertebral disc can be detected noninvasively by nuclear magnetic resonance (NMR) spectroscopy.
SUMMARY OF BACKGROUND DATA
Microbiological studies of surgical samples suggest that a significant subpopulation of back pain patients may have occult disc infection with P. acnes bacteria. This hypothesis is further supported by a double-blind clinical trial showing that back pain patients with Modic type 1 changes may respond to antibiotic treatment. Because significant side effects are associated with antibiotic treatment, there is a need for a noninvasive method to detect whether specific discs in back pain patients are infected with P acnes bacteria.
METHODS
P. acnes bacteria were obtained from human patients. NMR detection of a propionic acid (PA) in the bacteria extracts was conducted on 500 MHz high-resolution spectrometer, whereas in vivo NMR spectroscopy of an isolated bovine disk tissue infected with P. acnes was conducted on 7 T magnetic resonance imaging scanner.
RESULTS
NMR spectra of P. acnes metabolites revealed a distinct NMR signal with identical chemical shits (1.05 and 2.18 ppm) as PA (a primary P. acne metabolite). The 1.05 ppm signal does not overlap with other bacteria metabolites, and its intensity increases linearly with P. acnes concentration. Bovine disks injected with P. acnes bacteria revealed a very distinct NMR signal at 1.05 ppm, which linearly increased with P. acnes concentration.
CONCLUSION
The 1.05 ppm NMR signal from PA can be used as a marker of P. acnes infection of discs. This signal does not overlap with other disc metabolites and linearly depends on P. acnes concentration. Consequently, NMR spectroscopy may provide a noninvasive method to detect disc infection in the clinical setting.
LEVEL OF EVIDENCE
N/A.
研究设计
研究。
目的
本研究旨在探讨通过磁共振（NMR）光谱是否可以无创检测痤疮丙酸杆菌（P. acnes）对椎间盘的感染。
背景资料概要
手术样本的微生物研究表明，相当一部分背痛患者可能存在隐匿性椎间盘感染 P. acnes 细菌。这一假设进一步得到了一项双盲临床试验的支持，该试验表明，患有 Modic 型 1 改变的背痛患者可能对抗生素治疗有反应。由于抗生素治疗会带来严重的副作用，因此需要一种非侵入性的方法来检测背痛患者的特定椎间盘是否感染了 P acnes 细菌。
方法
从人类患者中获得 P. acnes 细菌。在 500 MHz 高分辨率光谱仪上对细菌提取物中的丙酸（PA）进行 NMR 检测，而在感染 P. acnes 的牛椎间盘组织的体内 NMR 光谱则在 7T 磁共振成像扫描仪上进行。
结果
P. acnes 代谢物的 NMR 光谱显示出与 PA（P. acne 的主要代谢物）相同化学位移（1.05 和 2.18 ppm）的独特 NMR 信号。1.05 ppm 信号不会与其他细菌代谢物重叠，其强度随 P. acnes 浓度呈线性增加。注入 P. acnes 细菌的牛椎间盘在 1.05 ppm 处显示出非常明显的 NMR 信号，该信号随 P. acnes 浓度呈线性增加。
结论
PA 的 1.05 ppm NMR 信号可用作椎间盘 P. acnes 感染的标志物。该信号不会与其他椎间盘代谢物重叠，并且与 P. acnes 浓度呈线性关系。因此，NMR 光谱可能为临床环境中检测椎间盘感染提供一种非侵入性方法。
证据水平
N/A。
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