Strzempko M N, Simon S L, French C K, Lippke J A, Raia F F, Savitt E D, Vaccaro K K
J Dent Res. 1987 Oct;66(10):1543-6. doi: 10.1177/00220345870660100601.
In this study, we evaluated the sensitivity and specificity of whole genomic DNA probes for the periodontal pathogens Haemophilus actinomycetemcomitans, Bacteroides intermedius, and Bacteroides gingivalis. By means of these probes, DNA hybridizations were performed against other organisms found in the oral cavity and organisms previously determined to be genetically similar. All three probes were sensitive to 10(3) cells for their respective organism. The H. actinomycetemcomitans probe cross-reacted with several haemophilus strains, Wolinella, and Campylobacter, indicating that H. actinomycetemcomitans-specific sequences would have to be identified and cloned for accurate detection of this organism in heterogeneous patient samples. Only very low levels of cross-reactivity were observed between the B. intermedius probe and representative black-pigmented Bacteroides. This low level of cross-reactivity did not interfere with the accurate identification of B. intermedius in sample evaluations. The B. gingivalis probe showed no cross-reactivity. Whole genomic probes will be used for the detection of B. intermedius and B. gingivalis in patient samples.
在本研究中，我们评估了全基因组DNA探针针对牙周病原体伴放线放线杆菌、中间普氏菌和牙龈卟啉单胞菌的敏感性和特异性。借助这些探针，对口腔中发现的其他微生物以及先前确定为基因相似的微生物进行了DNA杂交。所有三种探针对于各自对应的微生物对10³个细胞均敏感。伴放线放线杆菌探针与几种嗜血杆菌菌株、沃林氏菌属和弯曲菌发生交叉反应，这表明必须鉴定并克隆伴放线放线杆菌特异性序列，以便在异质患者样本中准确检测该微生物。中间普氏菌探针与代表性的产黑色素普氏菌之间仅观察到极低水平的交叉反应。这种低水平的交叉反应并不干扰在样本评估中对中间普氏菌的准确鉴定。牙龈卟啉单胞菌探针未显示交叉反应。全基因组探针将用于检测患者样本中的中间普氏菌和牙龈卟啉单胞菌。
