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使用合成寡核苷酸DNA探针鉴定牙龈卟啉单胞菌。

Use of synthetic oligonucleotide DNA probes for the identification of Bacteroides gingivalis.

作者信息

Moncla B J, Braham P, Dix K, Watanabe S, Schwartz D

机构信息

Department of Oral Biology, University of Washington, Seattle 98195.

出版信息

J Clin Microbiol. 1990 Feb;28(2):324-7. doi: 10.1128/jcm.28.2.324-327.1990.

DOI:10.1128/jcm.28.2.324-327.1990
PMID:1690217
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269599/
Abstract

Six different oligonucleotide probes complementary to the hypervariable regions of 16S rRNA of Bacteroides gingivalis were tested for specificity and sensitivity against 77 field strains of B. gingivalis and 105 strains of 12 other Bacteroides species. The data demonstrated that these probes were very specific (range, 0.85 to 1.00) and sensitive (1.00). Some limited cross-reactions with other Bacteroides species were observed. Four of these probes should be useful for rapid detection and identification of B. gingivalis.

摘要

针对牙龈卟啉单胞菌16S rRNA高变区的六种不同寡核苷酸探针，对77株牙龈卟啉单胞菌野外菌株以及12种其他卟啉单胞菌属的105株菌株进行了特异性和敏感性测试。数据表明这些探针具有很高的特异性（范围为0.85至1.00）和敏感性（1.00）。观察到与其他卟啉单胞菌属存在一些有限的交叉反应。其中四种探针可用于牙龈卟啉单胞菌的快速检测和鉴定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3b2/269599/22a2b6637f81/jcm00050-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3b2/269599/22a2b6637f81/jcm00050-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3b2/269599/22a2b6637f81/jcm00050-0176-a.jpg

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