Fundashon Prevenshon, Willemstad, Curaçao.
Department of Pathology, VU University Medical Centre, Amsterdam, the Netherlands.
Sex Transm Infect. 2018 Jun;94(4):263-267. doi: 10.1136/sextrans-2017-053109. Epub 2017 Oct 11.
Curaçao is a Dutch-Caribbean Island located in a high-risk area for cervical cancer.Prior to introduction of a prophylactic human papillomavirus (HPV) vaccine, knowledge of the prevalence of high-risk HPV vaccine genotypes (HPV16, 18, 31, 33, 45, 52 and 58) in cervical (pre)cancer is required.
To investigate the prevalence of HPV genotypes in invasive cervical cancers (ICC) and cervical intraepithelial neoplasia (CIN) grade 1, 2 and 3 in Curaçao.
Paraffin-embedded blocks of 104 cervical cancers (89 squamous, 15 adenocarcinoma), 41 CIN3, 39 CIN2 and 40 CIN1 lesions were analysed for the presence of HPV. Sections were stained by H&E for histopathological evaluation, and DNA was extracted using proteinase K. HPV genotypes were detected using Short PCR Fragment (SPF10) PCR DNA enzyme immunoassay and a Line Probe Assay (LiPA25) .
HPV was found in 92 (88.5%) ICC; 87 (94.6%) had a single HPV infection and 86 (93.5%) were high-risk human papillomavirus (hrHPV)-type positive.The three most common HPV types in ICC were 16 (38.5%), 18 (13.5%) and 45 (6.7%), covering 58.7%.HrHPV vaccine genotypes 16, 18, 31, 35, 45, 52 and 58 were responsible for 73.1% of ICC. For precancerous lesions, the HPV attribution was 85.4% for CIN3, 66.7% for CIN2% and 42.5% for CIN1.
Our study, the largest in the Caribbean region in (pre)cancer, shows that the prevalence of HPV-type 16 and 18 in cervical cancer is lower compared with the world population but no differences in prevalence of these two HPV types are seen in precancerous lesions.When considering HPV vaccination in Curaçao, the relatively high contribution of non-HPV 16/18 genotypes in ICC should be taken into account.
库拉索岛是位于宫颈癌高危地区的荷属加勒比岛屿。在引入预防性人乳头瘤病毒（HPV）疫苗之前，需要了解高危 HPV 疫苗基因型（HPV16、18、31、33、45、52 和 58）在宫颈（前）癌中的流行情况。
调查库拉索岛浸润性宫颈癌（ICC）和宫颈上皮内瘤变（CIN）1 级、2 级和 3 级中 HPV 基因型的流行情况。
对 104 例宫颈癌（89 例鳞癌，15 例腺癌）、41 例 CIN3、39 例 CIN2 和 40 例 CIN1 病变的石蜡包埋块进行 HPV 检测。使用 H&E 染色进行组织病理学评估，并使用蛋白酶 K 提取 DNA。使用短 PCR 片段（SPF10）PCR 酶免疫测定法和线性探针分析（LiPA25）检测 HPV 基因型。
ICC 中发现 HPV 92 例（88.5%）；87 例（94.6%）为单一 HPV 感染，86 例（93.5%）为高危型 HPV（hrHPV）阳性。ICC 中最常见的三种 HPV 类型为 16（38.5%）、18（13.5%）和 45（6.7%），占 58.7%。HPV16、18、31、35、45、52 和 58 型 hrHPV 疫苗占 ICC 的 73.1%。对于癌前病变，CIN3 的 HPV 归因率为 85.4%，CIN2%为 66.7%，CIN1 为 42.5%。
我们的研究是加勒比地区最大的（前）癌症研究，表明宫颈癌中 HPV 型 16 和 18 的流行率低于世界人口，但癌前病变中这两种 HPV 型的流行率没有差异。在考虑库拉索岛 HPV 疫苗接种时，应考虑到 ICC 中非 HPV16/18 基因型的相对高贡献。
