Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of electronic information and electrical engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.
Center for Advanced Electronic Materials and Devices, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.
Sci Rep. 2017 Oct 11;7(1):12967. doi: 10.1038/s41598-017-13389-x.
We report an innovative integrated microfluidic platform based on micro-fluxgate and micro-coils for trapping and detecting magnetic beads. A micro-spiral coil fabricated by microfabrication technology is used to trap the magnetic beads, and the micro-fluxgate is employed to detect the weak magnetic field induced by the trapped magnetic beads. The fabrication process of the magnetic bead trapping system using a micro-coil is highly compatible with that of the micro-fluxgate sensor, making fabrication of this integrated microfluidic system convenient and efficient. It is observed that the magnetic bead trapping ratio increases as the number of magnetic beads is increased with a flow rate of 5 to 16.5 μL·min. Samples spiked with different concentrations of magnetic beads can be distinguished clearly using the micro-fluxgate sensor in this microfluidic system. In this study, the results demonstrate that the microfluidic system traps and detects magnetic beads efficiently and is a promising candidate for biomarker capture and detection.
我们报告了一种基于微磁通门和微线圈的创新集成微流控平台，用于捕获和检测磁珠。通过微加工技术制造的微螺旋线圈用于捕获磁珠，而微磁通门则用于检测捕获磁珠引起的弱磁场。使用微线圈的磁珠捕获系统的制造过程与微磁通门传感器的制造过程高度兼容，使得这种集成微流控系统的制造既方便又高效。观察到，随着磁珠数量的增加，在 5 到 16.5 μL·min 的流速下，磁珠的捕获率增加。使用微流控系统中的微磁通门传感器可以清楚地区分不同浓度的磁珠样品。在这项研究中，结果表明微流控系统能够高效地捕获和检测磁珠，是生物标志物捕获和检测的有前途的候选者。
