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载多聚多巴胺仿生材料刺激骨髓间充质干细胞促进成骨效应。

Polydopamine-Laced Biomimetic Material Stimulation of Bone Marrow Derived Mesenchymal Stem Cells to Promote Osteogenic Effects.

机构信息

Oral and Craniofacial Health Sciences Research, School of Dentistry, University of North Carolina, CB #7455, Chapel Hill, NC, 27599, USA.

Department of Oral and Maxillofacial Surgery, School of Dentistry, University of North Carolina, CB #7454, Chapel Hill, NC, 27599, USA.

出版信息

Sci Rep. 2017 Oct 11;7(1):12984. doi: 10.1038/s41598-017-13326-y.

DOI:10.1038/s41598-017-13326-y
PMID:29021583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5636820/
Abstract

A hydroxyapatite-collagen (HC) composite material can mimic composition and ultra-structures of natural bone and provide adequate bioactive material-tissue interactions. Incorporation of dopamine (DA) is one of keys in increasing the mechanical strength of the HC material to approaching that of cortical bone. In this study, the in vitro osteogenic effects of polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA) were examined by culturing rat mesenchymal stem cells (rMSCs) on HCCS-PDA and HCCS coated plates. HCCS-PDA group demonstrated less cytotoxic from Live/Dead cytotoxic assay and displayed higher cell attachment, proliferation and mineralization than the HCCS group in vitro. For in vivo bone regeneration, HCCS-PDA or HCCS particulates with or without rMSC aggregates were implanted into rat critical-sized calvarial defects (CSD). After 12 weeks, calvarial bone regeneration was evaluated radiographically, histologically, and histomorphometrically. While the majority of new bone formation occurred around the HCCS-PDA particulates with rMSC aggregates, The HCCS-PDA particulates without rMSC aggregates showed limited osteoconductivity. HCCS with or without rMSC aggregates resulted in less bone formation, indicating a prominent role of DA in effective bone regeneration. Therefore, the HCCS-PDA biomaterial with the aid of rMSCs can be used to develop therapeutic strategies in bone tissue engineering with numerable clinical applications.

摘要

羟基磷灰石-胶原蛋白（HC）复合材料可以模拟天然骨的组成和超微结构，并提供足够的生物活性材料-组织相互作用。多巴胺（DA）的掺入是提高 HC 材料机械强度以接近皮质骨的关键之一。在这项研究中，通过在 HCCS-PDA 和 HCCS 涂层板上培养大鼠间充质干细胞（rMSCs），研究了聚多巴胺掺杂羟基磷灰石-胶原蛋白硅酸钙（HCCS-PDA）的体外成骨作用。与 HCCS 组相比，活/死细胞毒性试验显示 HCCS-PDA 组的细胞毒性较小，并且在体外表现出更高的细胞附着、增殖和矿化作用。对于体内骨再生，将 HCCS-PDA 或 HCCS 颗粒与或不与 rMSC 聚集物一起植入大鼠临界尺寸颅骨缺损（CSD）中。12 周后，通过影像学、组织学和组织形态计量学评估颅骨骨再生。虽然大多数新骨形成发生在含有 rMSC 聚集物的 HCCS-PDA 颗粒周围，但不含 rMSC 聚集物的 HCCS-PDA 颗粒表现出有限的骨诱导性。含有或不含有 rMSC 聚集物的 HCCS 导致骨形成较少，表明 DA 在有效骨再生中起重要作用。因此，具有 rMSCs 辅助的 HCCS-PDA 生物材料可用于开发具有无数临床应用的骨组织工程治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/311181137f17/41598_2017_13326_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/2c96040f36ae/41598_2017_13326_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/54bdac8a8f43/41598_2017_13326_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/0322772b856b/41598_2017_13326_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/409b6401bfab/41598_2017_13326_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/311181137f17/41598_2017_13326_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/2c96040f36ae/41598_2017_13326_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/93e0ea28bbce/41598_2017_13326_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/4a61ca791f79/41598_2017_13326_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/54bdac8a8f43/41598_2017_13326_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/0322772b856b/41598_2017_13326_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/27ea11680662/41598_2017_13326_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/409b6401bfab/41598_2017_13326_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/412d/5636820/311181137f17/41598_2017_13326_Fig8_HTML.jpg

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