Yamaoki Yudai, Kiyoishi Ayaka, Miyake Masayuki, Kano Fumi, Murata Masayuki, Nagata Takashi, Katahira Masato
Institute of Advanced Energy, Kyoto University, Kyoto 611-0011, Japan.
Phys Chem Chem Phys. 2018 Jan 31;20(5):2982-2985. doi: 10.1039/c7cp05188c.
In order to understand intracellular biological events, information on the structure, dynamics and interaction of proteins and nucleic acids in living cells is of crucial importance. In-cell NMR is a promising method to obtain this information. Although NMR signals of proteins in human cells have been reported, those of nucleic acids were reported only in Xenopus laevis oocytes, i.e., not in human cells. Here, DNA and RNA were introduced into human cells by means of pore formation by bacterial toxin streptolysin O and subsequent resealing. Then, NMR signals of DNA and RNA were successfully observed for the first time in living human cells. The observed signals directly suggested the formation of DNA and RNA hairpin structures in living human cells.
为了理解细胞内的生物学事件，关于活细胞中蛋白质和核酸的结构、动力学及相互作用的信息至关重要。细胞内核磁共振（In-cell NMR）是获取此类信息的一种很有前景的方法。尽管已报道了人类细胞中蛋白质的核磁共振信号，但核酸的核磁共振信号仅在非洲爪蟾卵母细胞中被报道过，即在人类细胞中未被报道。在此，通过细菌毒素链球菌溶血素O形成孔道并随后重新封闭的方式，将DNA和RNA导入人类细胞。然后，首次在活的人类细胞中成功观测到了DNA和RNA的核磁共振信号。观测到的信号直接表明在活的人类细胞中形成了DNA和RNA发夹结构。
